【摘要】 目的研究血管内皮生长因子-C(VEGF-C)及其血管内皮生长因子受体-3(VEGFR-3)在宫颈鳞癌组织中的表达及在淋巴道转移中的作用。方法采用免疫组化法检测62例宫颈鳞癌及宫颈上皮内瘤变组织中VEGF-C及VEGFR-3的表达,AE1/AE3检测盆腔淋巴结微转移。结果免疫组化AE1/AE3检测淋巴结转移检出率显著高于HE染色检出率;宫颈鳞癌中VEGF-C的阳性表达率与宫颈上皮内瘤变(CIN)Ⅰ、CINⅡ的阳性表达率差异有统计学意义(P<0.05),与CINⅢ无明显差异;VEGFR-3阳性淋巴管数显著高于宫颈上皮内瘤变;VEGF-C表达与VEGFR-3阳性淋巴管数呈明显正相关;VEGF-C及VEG-FR-3均与淋巴结的转移有关。结论AE1/AE3检查有利于发现淋巴结微转移;VEGF-C可能通过促进淋巴管的生成,从而利于宫颈鳞癌的浸润转移。更多还原

【Abstract】 Objective To study the expressions of VEGF-C and its receptor VEGFR-3 in cervical squamous cell carcinoma tissues,as well as their roles in lymph metastasis.Methods Sixty-two cases of cervical squamous cell carcinoma and cervical intraepithelial neoplasia were detected by immunohistochemical staining (S-P) for the expressions of VEGF-C,VEGFR-3 and tested the expression of AE1/AE3 in lymph node to explore lymph metastasis.Results The positive rates of AE1/AE3 by immunohistochemical staining in lymph nodes of cervical carcinoma were all more than those of routine H-E staining.The difference of positive expressive rate of VEGF-C between the cervical squamous cell carcinoma and CIN Ⅰ,CIN Ⅱ was statistically significant,but not to CINⅢ.The difference between the cervical squamous cell carcinoma and cervical intraepithelial neoplasia in VEGFR-3 expression was statistically significant.The expression level of VEGF-C in cervical squamous cell carcinoma was positively correlated with the positive number of lymphatic vessels stained by VEGFR-3.VEGF-C and VEGFR-3 were all related to lymph node metastasis.Conclusion The detection of AE1/AE3 may be much easier to find out the LN with micrometastasis;VEGF-C facilitates invasion and metastasis in cervical squamous cell carcinoma by promoting lymphangiogenesis.更多还原