【摘要】 目的机械分离并酶解小鼠耳蜗螺旋神经节神经元(SGN)细胞,观察其A型钾通道的电生理特性。方法对生后1～6d的小鼠耳蜗螺旋神经节组织进行机械分离并酶解,对SGN进行免疫细胞化学染色鉴定,并利用全细胞膜片钳技术记录和分析A型钾通道电流。结果新生小鼠SGN在机械分离和酶解条件下,可以获得良好的细胞形态。SGN的A型钾通道在-60mV时激活,+40mV时失活,能被细胞外的4-氨基吡啶阻滞。结论成功建立了机械分离并酶解SGN细胞的方法,SGN的A型钾通道具有激活快、失活快的特点。更多还原

【Abstract】 Objective To mechanically dissociate and enzymolize spiral ganglion neurons (SGN) in postnatal mice and to observe the electrophysiological characteristics of those A-type potassium channels.Methods The mechanical dissociation and enzymolysis of spiral ganglion neurons were carried out with 1-6 d postnatal mice.Immunocytochemical stain identification was performed with fluorescent microscope.Record and analysis of A-type potassium channels’ currents were carried out with whole-cell patch clamp techniques.Results Spiral ganglion neurons of postnatal mice had good cell forms in the condition of mechanical dissociation and enzymolysis.The A-type potassium channels were activated in the membrane voltage of-60mv and inactivated in the membrane voltage of +40mv,and were inhabited by extracellular 4-aminopyridine.Conclusion We successfully established the methods of mechanical dissociation and enzymolysis of SGN,the A-type potassium channels of SGN had the charactics of quick activation and quick inactivation.更多还原