【摘要】 目的探讨缺血后处理限制猪心肌梗死范围的作用。方法滇南小耳猪10只,随机分为2个组。实验组(n=5):在X线的监控下,经右股动脉置入冠状动脉球囊导管至左前降支,球囊扩张阻断血流60min,予8次球囊放气30s、充气30s短暂缺血再灌注即缺血后处理;对照组(n=5):不行缺血后处理,直接撤除球囊。测定缺血前、再灌注2h及24h心肌酶学指标。再灌注72h采用2,3,5氯化三苯基氯四氮唑(TTC)染色确定心肌梗死面积。结果再灌注后2h,两组的磷酸肌酸激酶(CK)及乳酸脱氢酶(LDH)较缺血前明显增高,差异有统计学意义(P<0.05);再灌注后24h,实验组的CK、LDH较对照组明显降低,差异有统计学意义(P<0.05)。病理学检查显示缺血后处理组梗死面积明显小于对照组,差异有统计学意义(P<0.05)。结论缺血后处理有缩小猪急性心肌梗死面积的保护作用。更多还原

【Abstract】 Objective To investigate the effects of ischemic postcondition on the reduction of infarction size in the swine model of acute myocardial infarction.Methods Ten Diannan small-ear pigs were divided into study group(n=5) and control group(n=5) randomly.A balloon catheter was positioned in the mid-distal left anterior descending (LAD) via right femoral artery under fluoroscopic guidance.The balloon was inflated and occluded LAD for 60 min.In study group,ischemic postconditioning was elicited by eight cycles of 30-s reperfusion,followed by 30-s reocclusion.Serum myocardial enzyme were assessed before ischemia,after 2 h of reperfusion,and after 24 h of reperfusion.After 72 h of reperfusion,infarction size was measured by 2,3,5-triphenyltetrazolium chloride(TTC) staining.Results After 2 h of reperfusion,the values of CK and LDH were higher than that before operation(P<0.05),after 24 h of reperfusion,the values of CK and LDH were lower than those in control group(P<0.05).In the study group,infarction size was smaller than that in control group(P<0.05).Conclusion Ischemic postcondition can reduce infarction size in the swine model of acute myocardial infarction.更多还原