【摘要】 目的:探讨22味中药对α-葡萄糖苷酶的抑制作用,筛选对α-葡萄糖苷酶抑制作用较强的中药。方法:以麦芽糖为底物,确定最优反应条件,以阿卡波糖为阳性对照,测定22味中药对α-葡萄糖苷酶的抑制作用。结果:22味中药对α-葡萄糖苷酶均有一定的抑制作用,抑制作用较强的有桑叶、桑白皮、地榆、青果和五倍子,浓度为500mg/mL时抑制率分别为63.19%、76.55%、70.03%、78.18%和92.83%,其中抑制作用最强的五倍子浓度为1000mg/mL时抑制率达到95.44%,与5mg/mL的阿卡波糖抑制作用相近。结论:桑叶、桑白皮、地榆、青果和五倍子5味中药体外对α-葡萄糖苷酶均有较强的抑制作用。更多还原

【Abstract】 Objective: To explore the inhibition of 22 kinds of Chinese herbs on α-glucosidase and screen Chinese herbs which have greater inhibition.Methods: The experiment was carried out with maltose as substrate,and then established optimal reaction conditions.The inhibition of 22 kinds of Chinese herbs and acarbose on α-glucosidase were tested by the enzyme reaction system.Here,acarbose was used as a positive control group.Results: All of the 22 kinds of Chinese herbs could inhibit the activity of α-glucosidase,and Sangye,Sangbaipi,Diyu,Qingguo and Wubeizi were the fi rst fi ve,the inhibition rates were 63.19%,76.55%,70.03%,78.18% and 92.83% respectively at the concentration of 500mg/ml.The inhibition of Wubeizi was the strongest,and when its concentration reached 1000mg/ml,the inhibition rate increased to 95.44%,which was similar to the effect of 5mg/ ml acarbose.Conclusion: Compared with the other Chinese herbs,Sangye,Sangbaipi,Diyu,Qingguo and Wubeizi had stronger inhibition on α-glucosidase in vitro.更多还原