【摘要】 目的探讨mPEG-SPA化学修饰的不同血型红细胞运用于临床主侧配血的适应性。方法先用mPEG-SPA化学修饰不同血型红细胞得到mPEG-SPA修饰红细胞,同时在红细胞修饰前后做定型试验以证实成功修饰;然后将mPEG-SPA修饰红细胞与40份不同血型的新鲜冰冻血浆和91份临床疑难配血标本分别用5种临床输血常用的配血方法(盐水法、间接抗球蛋白法、低离子介质间接抗球蛋白法、聚乙二醇间接抗球蛋白法和微柱凝胶卡法)进行主侧配血试验。结果 mPEG-SPA修饰红细胞与不同血型的合格新鲜冰冻血浆的主侧配合率为100%(40/40);与临床患者的疑难配血血清的主侧配合率为74.72%(68/91),明显高于这些疑难配血标本的临床配血配合率22.94%(312/1 360)(2χ=118.32,v=1,P<0.01)。结论采用mPEG-SPA化学修饰红细胞的方法将不同血型的红细胞变成通用的血型红细胞能够适用临床常用的主侧配血方法。更多还原

【Abstract】 Objectives To study the major cross-matching of blood with mPEG-SPA modified RBCs of different blood groups.Methods RBCs of different blood group were camouflaged into mPEG-SPA modified RBCs and blood group tests were performed to ensure a successful modification.The mPEG-SPA modified RBCs were then cross matched with 40 normal fresh frozen plasma,and 91 patients’ serum specimens containing antibodies using 5 commonly adopted methods,namely saline immediate spin,indirect antiglobulin test,low ionic strength saline-indirect antiglobulin test,polyethylene glycol indirect anti-globulin test and column agglutination technology.Results The mPEG-SPA modified RBCs were compatible with all 40 normal fresh frozen plasma,and with 74.72%(68/91) of the antibody containing patients′sera,while the latter were compatible with only 22.94%(312/1360) of random donor RBCs(χ2=118.32,v=1,P<0.01).Conclusion The universal RBCs obtained by mPEG-SPA modification of different blood group RBCs can be cross matched by methods commonly used in clinical practice.更多还原