【摘要】 检测患者乙型肝炎病毒前S1抗原(Pre-S1Ag)与抗-HBc IgM的水平,探讨两者与HBV急性感染的相关性,为乙型肝炎的早期诊治提供必要的理论依据。对90份临床标本分别采用PCR技术检测HBV-DNA载量阳性标本,采用固相R IA(SPR IA)检测HBeAg、抗-HBc IgM,采用胶体金免疫层析法检测Pre-S1Ag,并对结果进行分析。结果表明90份HBV-DNAPCR阳性标本中Pre-S1Ag阳性71份,HBeAg阳性60份,抗-HBc IgM阳性14份,其检测结果有显著性差异(P<0.05)。血清Pre-S1Ag、抗-HBc IgM及HBeAg与HBV复制紧密相关。Pre-S1Ag明显优于抗-HBc IgM及HBeAg。因此,Pre-S1Ag的检测对乙型肝炎临床早期诊断、抗病毒治疗方案的选择和预后判断具有重要意义。更多还原

【Abstract】 To explore the clinical value of detection of Hepatitis B virus Pre-S1 antigen(HBV Pre-S1Ag) and anti-HBc IgM level in early diagnosis of type B hepatitis patients,the HBV-DNA levels were detected by PCR,and HBeAg and anti-HBc IgM levels were measured by solid phase RIA,and the HBV Pre-S1Ag levels were detected by aurosol immunochromatographic method in 90 clinical specimens.The results showed that there were 71 Pre-S1Ag positive specimens,60 HBeAg positive specimens and 14 anti-HBc IgM positive specimens in all 90 HBV-DNA positive specimens.There was statistical significant difference between these different hepatitis B virus markers(P<0.05).The HBV replication was correlated with the serum HBV Pre-S1Ag,anti-HBc IgM and HBeAg.The serum Pre-S1Ag could reflect HBV replication better than anti-HBc IgM and HBeAg.The detection of serum Pre-S1Ag plays an important role in the early diagnosis of type B hepatitis,antiviral therapy plan selection and prognosis judgment.更多还原