【摘要】 提出了利用气相色谱-质谱联用仪同时定量测定植物组织中茉莉酸(JA)、吲哚-3-乙酸(IAA)和脱落酸(ABA)的方法。实验以欧洲灰杨叶片为材料,在用80%冷甲醇提取激素时,一并加入ABA、IAA和JA的内标(D3-ABA300ng,13C6-IAA60ng和DHJA80ng),激素提取液浓缩至水相,调pH2.5～3.0后经乙酸乙酯萃取、Sep-PakC18小柱纯化,甲酯化后,GC-MS定量测定ABA、JA。IAA经甲酯化后再经TMS化(三甲基硅烷化),用GC-MS定量测定。经检测:盆栽欧洲灰杨叶组织中脱落酸含量为120.170～128.771ng/g(鲜质量),茉莉酸含量为1.697～6.974ng/g(鲜质量),吲哚乙酸含量为4.990～14.776ng/g(鲜质量);脱落酸含量最高,其次是吲哚乙酸,茉莉酸含量最低。更多还原

【Abstract】 GC-MS was used to determine the concentrations of jasmonic acid(JA),indole-3-acetic acid(IAA) and abscisic acid(ABA) in Populus canescens leaves in this paper.Abundant cold methanol(80%,v/v) was used to extract JA,IAA and ABA,and three internal standards,D3-ABA 300 ng,13C6-IAA 60 ng and DHJA 80 ng were simultaneously introduced into the leaf samples.Crude extract was condensed by vacuum evaporating and hormones were re-extracted by ethyl acetate at pH 2.5-3.0(× 3).Then condensed JA,IAA and ABA extracts were purified by Sep-Pak C18 columns.Thereafter,partially purified hormones were methylated using trimethylsilyldizomethane and then analyzed by GC-MS(JA and ABA).IAA was analyzed by GC-MS after an additional TMS reaction.Results showed that concentrations of ABA,JA,and IAA in leaf tissues of P.canescens were 121.064-128.771 ng/g,2.190-6.974 ng/g and 4.990-15.012ng/g Fw,respectively.In concentration in P.canescens leaves,ABA > IAA > JA.更多还原