【摘要】 采用免疫酶的方法,研究了中药多糖对EB病毒早期抗原激活的抑制作用.结果表明:黄芪多糖、灵芝多糖、淫羊藿多糖在一定的剂量范围内均有其抑制作用,而且黄芪多糖、灵芝多糖的抑制作用明显优于淫羊藿多糖,三者都具有明显的量效关系.提示用这种方法可以在天然药物中寻找抗EB病毒药物,阻止其抗原的表达,防止细胞癌变,达到预防和治疗EB病毒相关性疾病的目的;同时也进一步揭示中药有效部位多糖的抗病毒、抗肿瘤的新机制.更多还原

【Abstract】 With the application of the immune enzyme assay,the inhibitory effects of herbal polysaccharides on the transcriptional activation of Epstein-Barr virus(EBV) early antigen in the Raji cell have been researched.The test result shows that Astragalus polysaccharide(APS),Ganoderma lucidum polysaccharide(GLP) and Epimedium polysaccharide(EPS) have inhibitory effects in certain range of dose.APS and GLP have significantly better inhibitory effects than EPS.In addition these polysaccharides and their inhibitory effects on EBV antigen expression have dose-effect relationships.In conclusion,this method can be used for finding new anti-EBV drugs in natural medicine.New anti-EBV drugs will inhibit EBV antigen expression and prevent cell carcinogenesis,so that they will be used for prevention and treatment of EBV associated disease.Furthermore the experiment reveals a new mechanism of herbal polysaccharides’ anti-virus and anti-tumor effects.更多还原