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中国柯萨奇病毒B5的全基因组测序及其序列分析

Complete Genome Sequence of Coxsackievirus B5(CVB5/09)Strain Isolated in China and Its Phylogenetic Analysis

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【作者】 胡永峰赵丽娜董杰吴志强杜江薛颖杨帆

【Author】 HU Yong-feng1,ZHAO Li-na2,3,DONG Jie2,WU Zhi-qiang2,DU Jiang2,XUE Ying2,YANG Fan2(1.State Key Laboratory for Molecular Virology and Genetic Engineering,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 100052,China;2.Institute of Pathogen Biology,Chinese Academy of Medical Sciences,Beijing 100176,China;3.Department of Biological Sciences of North China Coal Medical College,Tangshan,063000 China)

【机构】 中国疾病预防控制中心病毒病预防控制所病毒基因工程国家重点实验室中国医学科学院病原生物学研究所华北煤炭医学院生物科学系

【摘要】 本文对我国首株与手足口病相关的柯萨奇病毒B5(01/CVB5/SD/CHN/09,CVB5/09)进行了基因组测序并与现有的相关序列进行了比较和进化分析。CVB5/09基因组长7399nt,共编码氨基酸2185aa,与现有的CVB5基因组核酸序列相似性在80.6%~85.3%之间,氨基酸序列相似性在96.1%~96.9%。进化分析发现,利用不同的基因组片段P1、P2和P3区构建的进化树中,CVB5/09分别处在不同的进化分支上,不同基因组片段有着不同的进化速率。Simplot相似性分析没有发现基因组有明显的重组发生。本文完成了我国第1株柯萨奇病毒B5全基因组序列的测定,通过与其它相关病毒的比较分析深入了解其遗传特征,以期为手足口病的流行病学调查和预防控制提供有价值的信息。

【Abstract】 An outbreak of hand foot and mouth disease occurred in Shang dong,China in 2009.Almost 20% of patient’s swabs was positive for Coxsackie virus B5(CVB5)identified by RT-PCR and sequencing.It was suggested that CVB5 may be another important pathogen for HFMD.Fifteen pairs of overlapping primers were designed and the genome sequence was sequenced.The genome of CVB5 was 7 399 nt in length,coding for 2 185aa.The genome displayed 80.6%-85.3% nucleotide sequence identity and 96.1%-96.9% amino acid sequence identity with another three CVB5 respectively.Phylogenetic analysis showed that different segment of genome underwent a distinct evolutionary and selective pressure.Simplot analysis displayed no evident recombination between genome of CVB5 and other HEV B viruses.The complete and characterized genome of CVB5/09 provides further insight into the genetics of CVB5 and other HEV B viruses,aiding in the surveillance and control of HFMD.

  • 【文献出处】 病毒学报 ,Chinese Journal of Virology , 编辑部邮箱 ,2010年04期
  • 【分类号】R373.23
  • 【被引频次】18
  • 【下载频次】669
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