【摘要】 本文对我国首株与手足口病相关的柯萨奇病毒B5(01/CVB5/SD/CHN/09,CVB5/09)进行了基因组测序并与现有的相关序列进行了比较和进化分析。CVB5/09基因组长7399nt,共编码氨基酸2185aa,与现有的CVB5基因组核酸序列相似性在80.6%～85.3%之间,氨基酸序列相似性在96.1%～96.9%。进化分析发现,利用不同的基因组片段P1、P2和P3区构建的进化树中,CVB5/09分别处在不同的进化分支上,不同基因组片段有着不同的进化速率。Simplot相似性分析没有发现基因组有明显的重组发生。本文完成了我国第1株柯萨奇病毒B5全基因组序列的测定,通过与其它相关病毒的比较分析深入了解其遗传特征,以期为手足口病的流行病学调查和预防控制提供有价值的信息。更多还原

【Abstract】 An outbreak of hand foot and mouth disease occurred in Shang dong,China in 2009.Almost 20% of patient’s swabs was positive for Coxsackie virus B5(CVB5)identified by RT-PCR and sequencing.It was suggested that CVB5 may be another important pathogen for HFMD.Fifteen pairs of overlapping primers were designed and the genome sequence was sequenced.The genome of CVB5 was 7 399 nt in length,coding for 2 185aa.The genome displayed 80.6%-85.3% nucleotide sequence identity and 96.1%-96.9% amino acid sequence identity with another three CVB5 respectively.Phylogenetic analysis showed that different segment of genome underwent a distinct evolutionary and selective pressure.Simplot analysis displayed no evident recombination between genome of CVB5 and other HEV B viruses.The complete and characterized genome of CVB5/09 provides further insight into the genetics of CVB5 and other HEV B viruses,aiding in the surveillance and control of HFMD.更多还原