【摘要】 目的:构建一种复合型细胞外基质支架,并探讨其是否影响大鼠嗅鞘细胞(olfactory ensheathing cells,OEC)合成神经营养素。方法:将纤维蛋白原、层黏连蛋白和纤黏连蛋白按一定比例混合,在新鲜大鼠血浆促凝作用下,构建细胞外基质支架,在支架表面种植大鼠OEC,观察其生长增殖情况和对神经营养因子表达能力的影响。结果:OEC在复合支架上贴壁良好,增殖旺盛,伸出较长的突起。生长于复合支架上的OEC较玻璃片上的细胞合成更多的神经营养素。结论:细胞外基质支架能促进OEC增殖,同时促进OEC神经营养素分泌。更多还原

【Abstract】 Objective:To construct a kind of extracellular matrix scaffold and investigate whether it can influence the expression of neurotrophins in rat olfactory ensheathing cells.Methods:Mixed fibrinogen,laminin and fibronectin proportionally,then added fresh rat plasma to construct scaffold.Plated the rat olfactory ensheathing cells(OEC) on scaffold and then observed the influence of scaffold on proliferation and neurotrophin expression of OEC.Results:Rat OEC adhered to scaffold easily,proliferated quickly and extended long processes.Immunofluorescence and western blotting showed rat OEC on scaffold could produce more neurotrophins than those on glass.Conclusions:Extracellular matrix scaffold can promote proliferation of rat OEC and promote the production of neurotrophins.更多还原