【摘要】 采用组织分离法,以PDA培养基为分离培养基,从喜树的枝条、叶片、果实和根中分离获得28株内生真菌。对峙试验结果显示,28株内生真菌对6种植物病原菌均有不同程度的抑制作用;其中G-4菌株作用最明显,对苹果腐烂病菌(Valsa mali miyabeet Yamada)、茄褐纹病菌[Phomopsis vexans(Sacc.et Syd.)Harter]的抑制率分别为89.68%和83.88%。对G-4菌株生物学特性测定结果表明,G-4菌株生长最适温度为28℃,最适pH范围为6.4～8;在麦芽浸粉培养基上G-4菌丝生长迅速;果糖为最佳碳源。更多还原

【Abstract】 Taken on potato dextrose agaras as medium,28 strains of endophytic fungi were isolated from the stems,leaves,fruits and roots of Camptotheca acuminata Decne with tissue isolation method.The results of plat-confront indicated that 28 strains of endophytic fungi had some inhibition on the six plant pathogens;the strain G-4 has significant effect on pathogens,and the inhibition rates to Valsa mali and Phomopsis vexans were more than 89.68% and 83.88%,respectively.Biological characteristics of strain G-4 was studied,and the result showed that the strain G-4 grew well at 28℃ with pH 6.4～8;malt powder was the best medium for its growth,and fructose was the suitable carbon sources.更多还原