【摘要】 目的观察电针不同组合穴位(简称组穴)对围绝经期大鼠生殖内分泌免疫网络相关指标的影响以及不同组穴的差异性。方法将40只16月龄围绝经期大鼠随机分为围绝经期对照组、电针非经穴点组、电针1组(电针"关元"、"三阴交")和电针2组(电针"关元"、"三阴交"、"百会"、"风府"),另将10只4月龄大鼠设为青年对照组。电针刺激结束后,检测各组血清雌二醇(estradiol,E2)、卵泡刺激素(follicle sti mulating hormone,FSH)、黄体生成激素(luteinizing hormone,LH)、β-内啡肽(β-endorphin,β-EP)、白细胞介素-2(interleukin-2,IL-2)、去甲肾上腺素(norepi-nephrine,NE)及下丘脑NE、多巴胺(dopamine,DA)、5-羟色胺(5-hydroxytryptamine,5-HT)的含量;称取胸腺质量,计算胸腺指数。结果①与青年对照组比较,围绝经期对照组除血清NE外,各项指标均有显著性变化(P<0.05,或P<0.01)。②电针1组与围绝经期对照组比较,对血清E2、FSH、LH、NE,下丘脑DA、5-HT有显著调节作用(P<0.05,或P<0.01)。③电针2组与围绝经期对照组比较,对血清FSH、LH、IL-2、β-EP、NE,下丘脑NE、DA、5-HT有显著调节作用(P<0.05,或P<0.01);④电针2组与电针1组两组间比较,在对E2、FSH、LH、IL-2、胸腺指数的调节作用上存在显著性差异(P<0.05,或P<0.01)。结论电针不同组穴通过调节下丘脑-垂体-卵巢轴的功能治疗围绝经期综合征,且不同组穴的作用存在差异。更多还原

【Abstract】 Objective To observe effects of electroacupuncture(EA) of different acupoint combination on functions of reproduction-endocrine-immune network in perimenopausal rats,and compare the difference between two kinds of EA of acupoint combination.Methods Forty 16-month old Wistar rats during perimenopausal period were averagely randomized into perimenopausal control group,EA non-acupoints group,EA group 1(EA at "Neiguan" and "Sanyinjiao"),EA group 2(EA at "Guanyuan","Sanyinjiao","Baihui" and "Fengfu"),and another 10 four-month old rats were allocated into young control group.After EA stimulation,the serum levels of estradiol(E2),follicle-stimulating hormone(FSH),luteinizing hormone(LH),β-endorphin(β-EP),interleukin-2(IL-2) and norepinephrine(NE),and hypothalamic contents of NE,dopamine(DA) and 5-hydroxytryptamine(5-HT) were assayed.The thymus glands were collected for weighing and calculating the thymus index.Results(1)Compared with those in the young control group,all indicators had significant changes except for serum NE in perimenopausal control group(P<0.05,or P<0.01).(2)Compared with those in perimenopausal control group,the serum levels of E2,FSH,LH and NE,and the hypothalamic contents of DA and 5-HT had significant changes in EA group 1(P<0.05,or P<0.01).(3)Compared with perimenopausal control group,EA group 2 showed a significant regulation in the serum levels of FSH,LH,IL-2,β-EP and NE,and the hypothalamic contents of NE,DA and 5-HT(P<0.05,or P<0.01).(4)There were significant differeces in the serum levels of E2,FSH,LH and IL-2,and thymus index between EA group 1 and EA group 2(P<0.05,or P<0.01).Conclusion EA of different acupoint combination can alleviate perimenopausal syndrome by regulating the functions of hypothalamic-pituitary-ovarian axis,and the effects of one kind of acupoint combination are different from another.更多还原