【摘要】 目的探索复方牛苓颗粒治疗慢性非细菌性前列腺炎(chronic non-abacteriαl prostatitis,CNP)的作用机制。方法将72只SD雄性大鼠随机分为正常对照组,模型组,前列安通片组,复方牛苓颗粒高、中、低剂量组,每组12只。通过光镜观察各组大鼠前列腺组织形态学改变,用酶联免疫吸附法定量检测各组大鼠前列腺组织中肿瘤坏死因子-α(tumor necrosis factorα-,TNFα-)、白细胞介素-10(interleukin-10,IL-10)的表达水平。结果与正常对照组比较,模型组大鼠前列腺组织TNF-α水平显著升高(P<0.01)。与模型组比较,前列安通片组,复方牛苓颗粒高、中、低剂量组大鼠前列腺组织TNFα-含量显著降低(P<0.01),而IL-10水平显著升高(P<0.01)。与复方牛苓颗粒中、低剂量组比较,复方牛苓颗粒高剂量组大鼠前列腺组织TNFα-水平显著降低(P<0.01),IL-10水平显著升高(P<0.01)。CNP模型组大鼠前列腺组织有明显的慢性炎症表现,而复方牛苓颗粒高剂量组和前列安通片组大鼠前列腺组织的慢性炎症明显改善。结论细胞因子TNFα-、IL-10参与了CNP的炎症过程,复方牛苓颗粒通过升高前列腺组织IL-10水平和降低TNFα-水平,从而达到治疗CNP的作用。更多还原

【Abstract】 Objective To explore the mechanism of composite Niuling Granules in treating chronic non-bacterial prostatits(CNP).Methods A total of 72 SD rats were randomized into normal control group,model group,Qianlie Antong Tablets treated group,and high-,middle-,and low-dose composite Niuling Granules treated groups,with 12 rats in each group.The morphological changes of rat prostates were observed under light microscope;the levels of tumor necrosis factor α(TNF-α),and interleukin 10(IL-10) in the prostate tissues were assayed using enzyme linked immunosorbent assay.Results Compared with those in normal control group,the levels of TNF-α in the rat prostate tissues of model group were significantly increased(P<0.01).Compared with those of model group,the levels of TNF-α in the rat prostate tissues of Qianlie Antong Tablets treated group,and high-,middle-,and low-dose composite Niuling Granules treated groups were significantly decreased(P<0.01),while the levels of IL-10 were significantly increased(P<0.01).Compared with those of middle-and low-dose composite Niuling Granules treated group,the levels of TNF-α in the rat prostate tissues of high-dose composite Niuling Granules treated group were significantly decreased(P<0.01),while the levels of IL-10 were significantly increased(P<0.01).The prostate tissues of CNP model rats showed an obvious chronic inflammation,while the changes of chronic inflammation were alleviated obviously in the rat prostate tissues of groups treated with high-dose composite Niuling Tablets,and Qianlie AnTong Granules.Conclusion TNF-α and IL-10,as a pair of opposite cytokines,participate in the inflammation process of CNP.Composite Niuling Granules can treat CNP by increasing the levels of IL-10,and decreasing the levels of TNF-α in the rat prostate tissues.更多还原