【摘要】 目的研究细胞因子IFN-γ在小鼠不同妊娠阶段中的免疫调节作用。方法用ELISA检测不同孕期小鼠子宫和肝脏组织匀浆中IFN-γ的表达,通过免疫组织学方法观察IFN-γ清除对小鼠uNK细胞数量的影响。结果 ELISA检测后发现子宫分泌的IFN-γ与uNK细胞数的动力学变化特征呈伴随关系,并且其分泌量均低于同期肝脏的分泌水平。由于IFN-γ对uNK细胞的扩增有重要的作用,IFN-γ清除鼠的uNK细胞数量明显少于对照鼠。结论子宫NK细胞有大量表达IFN-γ的潜能,正常情况下仅适量分泌以维持Th1/Th2之间的平衡,保证妊娠的顺利进行。更多还原

【Abstract】 Objective To compare the differences of cytokines secretion of IFN-γ by NK cells in uterine (uNK cells)and different organ-derived murine NK cells,and focus on the cytokines secretion by uNK cells.Methods Mechanical dissection approaches were used to prepare cell suspension.The cytokines production of IFN-γ in different tissues during pregnancy were detected by enzyme-linked immunosorbent assay (ELISA)? Histological study was also conducted to confirm the uNK population after anti-IFN-γ blocking,by periodic acid-Schiff (PAS) staining?Results It was demonstrated that the expression of IFN-γ in uterine endometrium of pregnant mice were lower than in liver,but increased significantly during pregnancy till mid gestation day? Uterine NK cells had more IFN-γ production ability than other organ-derived NK cells? Anti IFN-γ treatment decreased the local uNKcells number in pregnant mice.Conclusion Uterine NK cells have a potential of producing abundant IFN-γ,but a few cytokines were produced in order to banlance the content of Th1/Th2 type cytokine during the normal pregnancy of mice,thus ensuring successful pregnancy progress.更多还原