【摘要】 [目的]研究用基质固相分散法提取净化,离子色谱法测定卷烟中的糖和多元醇。[方法]将样品与石墨化碳黑球(W/W,1∶4)充分研磨后装柱,用热水洗脱,洗脱液中的糖用离子色谱测定。以CarboPac MA1(4×250 mm,5μm)阴离子交换柱为固定相,0.5 mol/LNaOH溶液为流动相分离,脉冲安培检测器检测。[结果]一次进样可实现卷烟样品中甘油、丙二醇、木糖醇、山梨醇、葡萄糖、果糖、蔗糖、麦芽糖和半乳糖9种成分的同时测定。方法加标回收率在94.6%~105.7%,相对标准偏差小于3.0%。检测限均小于50μg/L。[结论]该方法重现性好,准确可靠,回收率较好,可用于实际烟草样品中糖和多元醇的测定。更多还原

【Abstract】 [Objective] The aim was to determine the sugars and polyols in tobacco based on matrix solid phase dispersion.[Method] The samples and graphitized carbon black sorbent(1∶4) were homogenized in a mortar and then packed into a cartridge.The sugars and polyols were eluted from the cartridge with water.Then,they were analyzed by ion chromatography with integrated pulsed amperometric detector.Taking CarboPac MA1(4×250 mm,5 μm) anion exchange column as stationary phase,0.5 mol/L sodium hydroxide solution as mobile phase,and pulsed amperometric detector was used.[Results] The 9 components of glycerol,propanediol,xylitol,sorbitol,glucose,fructose,sucrose,maltose,galactose were separated at the same as by that method.The limits of detections(S/N=3) were below 50 μg/L.The mean recoveries were range from 94.6%-105.7%,and the relative standard deviations were below 3.0%.[Conclusion] This method is good reproducibility and recovery rate,accurate and reliable,it can be applied to the determination of sugar and polyols in tobacco.更多还原