【摘要】 [目的]通过组织培养方法筛选枫叶秋海棠不定芽诱导、伸长及生根的最佳培养基,为枫叶秋海棠的快速繁殖提供参考。[方法]以枫叶秋海棠的叶柄、叶片为外植体,进行不定芽诱导、伸长及生根的组织培养。[结果]枫叶秋海棠叶片最佳芽诱导、芽伸长培养基为MS+ZT(2.0mg/L)+NAA(0.2mg/L)。生根的最佳培养基为1/2MS+IBA(0.2mg/L),试管苗移栽后成活率为100%。[结论]该试验建立了枫叶秋海棠组织培养快速繁殖体系,为枫叶秋海棠的快速繁殖和遗传转化提供理论依据。更多还原

【Abstract】 [Objective] The study aimed to screen the optimum medium for the induction elongation of adventitions bud,and rooting of Begonia heracleifoniana and provide reference for the rapid propagation of Begonia heracleifoniana.[Method] With leaves and petiole of Begonia heracleifolia as explants,the culture condition of the induction of adventitions bud and rooting were studied.[Result] The optium medium for the differentiation and elongation of leaves from Begonia heracleifonia was MS+ZT(2.0 mg/L)+NAA(0.2 mg/L).The optimum medium for the rooting of it was 1/2MS+IBA(0.2 mg/L),and the survival rate of test tube seedling was 100% after being transplanted.[Conclusion]The rapid propagation system of Begonia heracleifoniana was established in this study,which would provide theoretical evidence for the rapid propagation and genetic transformation of Begonia heracleifoniana.更多还原