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60Co-γ辐射对马铃薯耐盐愈伤组织诱导的影响
Effects of 60Co-γ Radiation Treatment on Inducing Potato Salt Tolerant Callus
【摘要】 [目的]为马铃薯辐射诱变育种研究提供科学依据。[方法]测定不同剂量60Co-γ辐射与不同浓度NaCl对马铃薯愈伤组织生长的影响,并在组织培养过程中定向筛选耐盐变异体。[结果]在辐射处理与盐胁迫的双重作用下,马铃薯的愈伤组织发生了一定程度的变异,使得愈伤组织对盐胁迫的耐受力有了大幅度的提高。[结论]当辐射剂量为40J/kg,NaCl浓度为2.0%时,对马铃薯愈伤组织的诱导效果最好。
【Abstract】 [Objective]The study aimed to provide the scientific basis on the research of irradiation breeding of potato.[Method]The effects of different dose 60Co-γ radiation and different concentration NaCl on potato’s callus tissue were determined,and salt-tolerant variant was screened during the process of tissue cultivation.[Result]Under the dual function of radiation treatment and salt stress,the callus of potato showed a certain extent variation,and it made salt tolerance of callus showed a greatly rise.[Conclusion] The induction effect on potato callus reached the best when 40 J/kg radiation and 2.0% NaCl.
- 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2010年12期
- 【分类号】S532
- 【被引频次】17
- 【下载频次】135