【摘要】 Harpins是由革兰氏阴性植物病原细菌产生的一类蛋白激发子,对蛋白酶敏感,在非寄主植物叶片上可以诱导hypersensitive response(HR)或hypersensitive cell death(HCD)。HrpNEa是Harpins蛋白的一种,是从梨火疫病菌(Erwinia amylovora)中分离得到的。大量试验证明,HrpNEa蛋白在引起植物过敏反应的同时,能诱导植物获得性系统抗性及刺激植物生长发育。从分子生物学角度,利用国际互联网上的相关生物信息学分析软件NPSA、Swiss-Model、SAPS、InterPro Scan等对HrpNEa蛋白进行了结构和功能的预测分析。HrpNEa蛋白由403个氨基酸组成,有较多的β折叠和无规卷曲结构;HrpNEa蛋白无N端信号肽,不形成跨膜结构,定位于细胞质中。更多还原

【Abstract】 Harpins produced by gram-negative plant pathogenic bacteria,a class of elicitor proteins,can induce hypersensitive response(HR) or hypersensitive cell death(HCD) in non-host plant leaves.HrpNEa is one kind of Harpins.It is separated from Erwinia amylovora.A large number of experiments proved that HrpNEa causes hypersensitive reactions in plants,also can induce acquired systemic resistance of plants and stimulate plant growth and development at the same time.The study analyzed protein structure and function of HrpNEa from the perspective of molecular biology by using bioinformatics software such as NPSA,Swiss-Model,SAPS,InterPro Scan and so on.HrpNEa is composed of 403 amino acids,and it has more β-sheet and random coil structure;HrpNEa protein without N-signal peptide,does not form a transmembrane structure,located in the cytoplasm.更多还原