【摘要】 [目的]研究重金属铅对鲫鱼抗氧化能力的影响。[方法]用0、0.01、0.05、0.10、0.50、1.00 mmol/L的含铅水溶液处理鲫鱼,比较不同处理鲫鱼血清过氧化氢含量、过氧化氢酶和髓过氧化物酶活力。[结果]低浓度铅(0.01 mmol/L)对鲫鱼血清CAT活力具有一定的促进作用,高浓度铅对鲫鱼血清CAT活力具有抑制作用;鲫鱼血清中H2O2含量随铅离子处理浓度的升高呈上升趋势,但1.00 mmol/L铅离子处理组鲫鱼血清H2O2含量低于0.50 mmol/L铅离子处理组;较低浓度的铅(0.01、0.05 mmol/L)能够提高鲫鱼血清MPO活力,高浓度铅则抑制鲫鱼血清MPO活力。[结论]该研究为鱼类生存环境重金属污染的定量分析提供了参考。更多还原

【Abstract】 [Objective] The aim was to study the effect of Pb on antioxidant capacity of Carassius auratus.[Method] The C.auratus were treated by Pb solution with 0,0.01,0.05,0.10,0.50,1.00 mmol/L,the content of hydrogen peroxide(H2O2),activities of catalase(CAT) and myeloperoxidase(MPO) in serum of C.auratus in different treatments were compared.[Result] Pb with low concn.(0.01 mmol/L) could increase the activity of CAT in serum of C.auratus,while Pb with high concn.could decrease the activity of CAT.H2O2 content in serum of C.auratus showed increasing trend with the increase of Pb concn,but H2O2 content in serum of C.auratus in treatment with 1.00 mmol/L Pb was lower than that in treatment with 0.50 mmol/L Pb.Pb with low concn.(0.01,0.05 mmol/L) could increase the activity of MPO in serum of C.auratus,while Pb with high concn.could decrease the activity of MPO.[Conclusion] The study provided the reference for quantitative analysis of heavy mental pollution in living environment of fish.更多还原