【摘要】 [目的]探讨不同重组大肠杆菌菌株保存的方法,找出其保存的最适条件。[方法]以携带质粒pcDNA3的大肠杆菌DH5α为研究对象,分析了4℃冷藏和-70℃冷冻保存对菌株活性的影响。[结果]4℃条件下,高稀释度的菌液中活细胞数随着时间的延长而逐渐上升,到第7天时达到最高峰,随后呈现逐渐下降的趋势-70℃条件下,菌液OD600值为0.8,甘油终浓度为8%的处理组,在各保存时间段的活细胞数均显著高于其他组。[结论]4℃短期保存重组菌株时,菌液最佳存放时间为7 d左右;-70℃长期保存时,宜采用对数生长期的细菌,且保持甘油工作浓度8%为宜。更多还原

【Abstract】 [Objective] To explore different preservation methods of recombinant E.coli and find out the optimal conditions for preservation.[Method] The recombinant E.coli DH5α transformed pcDNA3 were respectively preserved at 4 ℃ and-70 ℃,and the activity was determined after different time.[Result] The number of living E.coli with high dilutions preserved at 4 ℃ was gradually increased within the first 7 d,peaked on Day 7,and then gradually decreased.The number of living E.coli,which were preserved in 8% glycerol at-70 ℃ when OD600 at 0.8,were significantly higher than that of other groups after different preservation time.[Conclusion] The optimal storage time was 7 d for recombinant E.coli at 4 ℃.For preservation at-70 ℃,the bacteria should be at logarithmic growth phase and preserved in 8% glycerol.更多还原