【摘要】 目的:了解松塔球提取物的抑突变作用。方法:不同剂量(50、250和500mg/kg)的松塔球碱性提取物溶液与环磷酰胺(50mg/kg)联用,连续4d腹腔注射昆明小鼠,取骨髓制片,计数含微核的骨骼嗜多染红细胞。结果:50mg/kg松塔球提取物溶液和环磷酰胺联用时与环磷酰胺组比较微核率差异无统计学意义(P>0.05);松塔球提取物250和500mg/kg剂量与环磷酰胺联用组微核率明显低于环磷酰胺组(P<0.05),其微核抑制率分别为22.35%、65.35%。雌性和雄性小鼠组间微核率差异无统计学意义(P>0.05)。结论:松塔球提取物可有效抑制环磷酰胺诱导的小鼠骨髓细胞微核的产生,并且存在明显的剂量_反应关系,具有抑突变性,提示松塔球提取物对肿瘤的防治可能具有积极的作用。更多还原

【Abstract】 OBJECTIVE:To investigate the anti-mutation effect of the extracts of pinecones.METHODS:The anti-mutation effect of extracts of pinecones was measured by micronucleus test,with different doses of pinecones alkaline extract solution(50、250 and 500 mg/kg) as samples,cyclophosphamide as the positive group.RESULTS:The mice marrow cells MN rate of high,middle and low doses of extract solution plus cyclophosphamide treatment groups:16‰±0.58‰,34.75‰±0.85‰ and 40.25‰±0.48‰,respectively.Compared with physiological saline group the difference in MN rate was statistically significant (P<0.01).For extract solution in low dose group compared with CP group,the difference was not statistically significant (P>0.05).In the high and middle dose groups,the difference was statistically significant (P<0.05),inhibition rates were 22.35% and 65.35%,respectively.Between male and female mices the difference was not statistically significant (P>0.05).CONCLUSION:The extracts of pinecones could suppress mice marrow cells induced by cyclophoshomide,with an obvious dose-response relation.It also showed anti-mutation effects.The extracts of pinecones may have positive effect on tumor prevention.更多还原