【摘要】 目的研究增龄进程中大鼠淋巴细胞中核转录因子κB(NF-κB)信号通路跨膜受体的mRNA水平差异表达特征,以及淫羊藿总黄酮(EF)对其的干预效应。方法将SD大鼠随机分为3天、4月龄、10月龄、18月龄、27月龄、27月+EF,以大鼠脾脏分离的淋巴细胞为研究对象,定制NF-κB信号通路基因芯片,分别检测各组NF-κB信号通路上游跨膜受体mRNA水平的整体表达特征及差异,并观察EF对NF-κB相关基因的干预效应。结果Tgfbr2、Tlr7、Ripk2出生3天表达最低,4或10个月达峰值,之后渐低,至27个月降至最低,EF干预后Ripk2变化较明显,接近10个月水平;Tnfrsf1a与Tlr2在3天时达峰,4个月骤然降低,而后在10、18、27个月均维持在一相近的水平,但经EF作用后有显著升高;Tnfrsf5自3天至18个月逐渐升高,但至27个月骤然下降,EF作用后略有回升;Madh4表达比较特殊,在4个月最高,10个月最低,18个月显著升高,至27个月再次降低,EF干预后略有提高,但变化不明显;Tnfrsf10b、Il1r1、Tlr1、Tlr4在增龄进程中的变化不明显。结论淫羊藿总黄酮的提前干预可对增龄进程淋巴细胞NF-κB信号通路跨膜受体基因mRNA表达产生一定的良性影响,尤其是对Ripk2的作用最为显著,这可能是EF作用的重要靶点之一。更多还原

【Abstract】 Objective To study the lymphocyte NF-к B signal passage cross membrane acceptor mRNA expression characteristics of rats in increasing age and the intervention effect of total flavone of Epimedium(EF).Methods The SD rats were randomized into 6 groups: 3 days(3d),4 months(4m),10 months(10m),18 months(18m),27 months(27m),27m+EF.The lymphocytes separated from the spleen of rats were taken as the object and the NF-кB signal passage gene chip was used to examine the expression characteristics and differences of NF-кB signal passage upstream cross membrane acceptor mRNA of each group and to observe intervention effect of EF on NF-κB related gene.Results The expression of Tgfbr2,Tlr7,Ripk2 of 3d was the lowest,reached the peak 4m or 10m,afterward gradually lowered,dropping to the lowest 27m,with EF intervention,Ripk2 changed obviously,close to 10m level;Tnfrsf1a and Tlr2 reached the peak on the 3d,in the 4m reduced suddenly,then in 10m,18m and 27m maintained at a similar level,but with the EF intervention they were obviously increased;Tnfrsf5 increased gradually from 3d to 18m,but dropped suddenly in 27m,with EF intervention,it was slightly increased;the Madh4 expression was quite special,to the highest in 4m,to the lowest in 10m,increased obviously in 18m,reduced again in 27m,with EF intervention,slightly increased;the change of Tnfrsf10b,Il1r1,Tlr1,Tlr4 in increasing age was not obvious.Conclusion In the senile advancement,lymphocyte NF-κB signal passage cross membrane acceptor mRNA increasing age dependence expression regulation characteristics were not inconsistent,the EF intervention produces a certain good influence on the old age group for gene mRNA expression,particularly a remarkable effect on Ripk2.This is possibly one of important targets of EF functions.更多还原