【摘要】 目的探讨自发性高血压大鼠(spontaneous hypertensive rat,SHR)肥厚心肌局部脂联素信号转导分子的改变,并观察红景天和灵芝孢子粉对其血流动力学和脂联素信号转导分子的影响。方法以8周龄WKY大鼠为对照,将8周龄SHR大鼠随机分为SHR组、红景天组、灵芝孢子粉组以及红景天和灵芝孢子粉联合组,连续灌胃4周。观察期满后,用颈总动脉插管法测量血流动力学指标;ELISA试剂盒检测血清脂联素水平;RT-PCR检测心肌脂联素受体(AdipoR)及肉毒碱棕榈酰转移酶(CPT-1)mRNA的表达;Western blot检测心肌磷酸化和非磷酸化腺苷酸活化蛋白激酶(AMPK)的蛋白表达。结果与WKY大鼠比较,SHR大鼠收缩压、舒张压和心肌肥大指数均显著升高,血清脂联素、心肌组织AdipoR1、CPT-1mRNA和磷酸化AMPK含量降低(P<0.05)。与SHR大鼠比较,红景天及灵芝孢子粉单独或联合应用都可显著降低收缩压、舒张压及心肌肥大指数(P<0.01,P<0.05),增高CPT-1mRNA表达(P<0.05);但血清脂联素、心肌组织AdipoR1和磷酸化AMPK含量只在红景天及灵芝孢子粉联合应用时才有升高(P<0.05)。结论SHR大鼠肥大心肌中脂联素信号转导通路发生变化,这可能是导致肥大心肌代谢改变的分子机制之一。应用红景天及灵芝孢子粉可改善血流动力学指标,增加脂联素及其相关信号转导分子的表达。更多还原

【Abstract】 Objective To investigate the alteration of the adiponectin signal pathway in hypertrophic myocardium of spontaneous hypertensive rats(SHR)and to observe the effects of Gadol(GD)and Ganoderma spores(GS)on the hemodynamic parameters and the adiponectin signal pathway of SHR.MethodsSHRs,8 weeks old,were randomly divided into four groups:the untreated group,and the three treated groups treated with GD,GS,and GD+GS respectively by gastrogavage for 4 weeks.Controlled with 8-week-old WKY rats,the hemodynamic parameters in all rats were recorded through the carotid artery intubation;the serum level of adiponectin was determined with ELISA;the mRNA expressions of adiponectin receptors(AdipoRs)and carnitine palmitoyl transferase(CPT-1)were determined by RT-PCR;and the protein expression of adenosine monophosphate activated protein kinase(AMPK),both phosphorylated and un-phosphorylated,was detected by Western blot.ResultsCompared with the WKY rats,the systolic blood pressure(SBP),diastolic blood pressure(DBP)and myocardial hypertrophy index(MHI)in SHR were significantly higher;the serum levels of adiponectin and phosphorylated AMPK,mRNA expressions of AdipoR1 and CPT-1 in SHR heart tissue were lower(P<0.05).Compared with the SHR,medication of GD and GS,either alone or in combination,could reduce SBP,DBP and MHI significantly(P< 0.01,P< 0.05),and elevate the mRNA expression of CPT-1(P<0.05)in heart,but levels of adiponectin,AdipoR1 and phosphorylated AMPK could only be raised by combined use of the two(P<0.05).ConclusionsAdiponectin signal transduction pathway alteration presents in the myocardium of SHR,which might be one of the molecular mechanisms that cause hypertrophic metabolic abnormality.GD and GS could improve the hemodynamic index in SHR,and enhance the level of adiponectin and the expression of its related signal transduction molecules.更多还原