【摘要】 目的探讨丹参酮ⅡA(TSN)对血管紧张素Ⅱ(AngⅡ)诱导血管平滑肌细胞(VSMCs)增殖的影响机制。方法组织贴块法培养VSMCs并用AngⅡ诱导建立其增殖模型,用酶促反应定磷法观察不同浓度的TSN对血管平滑肌细胞中钙调神经磷酸酶(CaN)活性的影响;应用逆转录聚合酶链式反应(RT-PCR)观察CaN mRNA表达水平的变化和应用免疫细胞化学方法观察增殖细胞核抗原(PCNA)表达水平的变化。结果与正常对照组比较,AngⅡ组能够明显刺激VSMCs增殖,细胞增殖活度明显升高(P<0.05);TSN各组CaN活性、CaN mRNA和PCNA表达水平随TSN的浓度增加而显著下降(P<0.05,P<0.01)。结论TSN具有抑制血管平滑肌细胞增殖的作用,并呈浓度依赖性。其机制可能与TSN下调VSMC中CaN活性、抑制了CaN mRNA和PCNA的表达有关。更多还原

【Abstract】 Objective To study the effect of tanshinone ⅡA(TSN) on angiotensin Ⅱ(AngⅡ) induced proliferation of vascular smooth muscle cells(VSMCs).MethodsVSMCs were cultured by explant attached method,and induced to proliferative cell model with AngⅡ.The effect of TSN in different concentrations on calcineurin(CaN) activity was detected by enzyme reaction phosphorus measurement;the CaN mRNA expression was detected by RT-PCR;and the expression of proliferating cell nuclear antigen(PCNA) were observed by immunocytochemical method.ResultsCompared with the normal control group,AngⅡ could significantly stimulate the proliferation of VSMCs,showing obviously elevated degree of proliferation activity(P<0.01).After being treated with TSN,all the indexes,including CaN activity,CaN mRNA expression and PCNA expression,were obviously reduced in a dose-dependent manner(P<0.05,P<0.01).ConclusionVSMCs proliferation can be inhibited by TSN in a dose-dependent manner and the inhibiting mechanism may be related to the down-regulation of CaN activities and the inhibition on CaN mRNA and PCNA expressions.更多还原