【摘要】 目的:探讨线粒体融合蛋白2(mitofusin 2,Mfn2)在非小细胞肺癌(non-small-cell lung cancer,NSCLC)组织和非癌肺组织中的表达及与临床病理特征之间的关系。方法:应用原位杂交和免疫组织化学法检测92例NSCLC组织和27例非癌肺组织中Mfn2 mRNA及蛋白的表达。结果:肺鳞癌组织中Mfn2 mRNA和蛋白的阳性表达率(83.33%和89.58%)均高于肺腺癌(56.82%和65.91%),且2者均高于非癌肺组织的阳性表达率(25.93%和29.63%),3组间比较差异有统计学意义(P<0.001和P<0.01)。高分化和中分化的肺癌组织中Mfn2 mRNA和蛋白的阳性表达率(93.75%和100%,91.67%和91.67%)均高于低分化的肺癌组织(21.43%和42.86%),差异有统计学意义(P<0.001)。Mfn2 mRNA和蛋白的表达与患者的性别、年龄、肿瘤大小、TNM分期和淋巴结转移无关(P>0.05)。肺癌组织中Mfn2 mRNA和蛋白的表达具有较好的一致性。结论:Mfn2参与了肺癌的发生和发展,Mfn2的表达与肺癌的组织学类型及分化程度有关。更多还原

【Abstract】 Objective:To investigate the expressions of Mfn2(mitofusin 2) in non-small-cell lung cancer(NSCLC) tissues and non-cancerous lung tissues,and analyze its relationship with clinicopathological characteristics of lung carcinomas.Methods:The expressions of Mfn2 mRNA and protein in 92 cases of NSCLC tissues and 27 cases of non-cancerous lung tissues were detected by in situ hybridization and immunohistochemical methods.Results:The positive rates of Mfn2 mRNA and protein in pulmonary squamous cell carcinoma were higher than those in adenocarcinoma(83.33% and 89.58% vs 56.82% and 65.91%),respectively.The positive rates of Mfn2 mRNA and protein in NSCLC were higher than those in the non-cancerous lung tissues(25.93% and 29.63%).The difference was statistically significant among the three groups(P<0.001 and P<0.01).The expressions of Mfn2 mRNA and protein in well-differentiated(93.75% and 100%) and moderately-differentiated NSCLC(91.67% and 91.67%) were higher than those in poor-diffe-rentiated NSCLC(21.43% and 42.86%).The difference was significant(P<0.001).The expressions of Mfn2 mRNA and protein had no correlation with the gender,age,tumor size,TNM stage and lymph node metastasis(P>0.05).The expression of Mfn2 mRNA was consistent with that of Mfn2 protein in NSCLC.Conclusion:Mfn2 was involved in the initiation and progression of lung cancer,and the expression of Mfn2 was related to the histological types of lung cancer and its differentiation degree.更多还原