【摘要】 目的:探讨细胞周期和凋亡相关蛋白bcl-2和bax与脑胶质瘤病理分级的相关性,为脑胶质瘤严重程度的临床评价和机制研究提供参考依据。方法:取99例脑胶质瘤组织,分离获得单个胶质瘤细胞,采用FCM进行细胞周期和bcl-2及bax的测定,同时收集相应的病理分级资料,进行相关性分析和多元逐步回归分析。结果:病理分级与细胞周期(S期)及bcl-2、bcl-2/bax均呈正相关性,随着脑胶质瘤病理分级程度的升高,S期比例上升(t=2.48,P=0.018 4,R2=0.90),bcl-2、bcl-2/bax比值均上升(t=23.26,P<0.000 1,R2=0.93;t=27.42,P<0.000 1,R2=0.85)。结论:细胞周期S期分析和bcl-2、bcl-2/bax在脑胶质瘤严重程度的临床评价和机制研究中具有一定的参考价值。更多还原

【Abstract】 Objective:To study the relationship between cell cycle,bcl-2 and bax expressions and the pathological grades of glioma in order to provide help for clinical evaluation and mechanism research of the severity of human glioma.Methods:This study collected 99 human glioma samples and isolated single glioma cells.Flow cytometry was used to detect their cell cycle and determine the expression levels of bcl-2 and bax.The pathological grading data were collected concurrently.The correlation and multiple stepwise regression analysis were analyzed by SAS software.Results:Pathologic grades had positive correlation with the percentage of glioma cells in S phase,the level of bcl-2,and the value of bcl-2/bax.As the increase in the pathologic grade,the percentage of glioma cells in S phase was also increased(t=2.48,P=0.018 4,R2=0.90),the expression level of bcl-2 and the ratio of bcl-2/bax were elevated(t=23.26,P<0.000 1,R2=0.93;t=27.42,P<0.000 1,R2=0.85).Conclusion:The percentage of glioma cells in S phase,the level of bcl-2,and the ratio of bcl-2/bax had some value for clinical evaluation and mechanism research of severity of glioma.更多还原