【摘要】 为了提高丝素材料的抗凝血性能,尝试用活血化淤中草药川芎的有效成分川芎嗪对其进行改性.首先对川芎嗪的侧链进行结构修饰引入活性基团羟基,然后通过酯化反应引入双键,将得到的中间体进一步在无引发剂条件下接枝聚合在丝素材料表面.用傅立叶转换红外光谱(FTIR)、傅里叶变换衰减全反射红外光谱(ATR-FTIR)、扫描电镜(SEM)对改性丝素进行了结构鉴定.改性丝素的抗凝血性能通过部分凝血活酶时间(APTT)和血小板粘附等实验进行评价,和未改性丝素相比,改性后的丝素APTT时间延长,同时在富血小板血浆中培养30 min后未发现有粘附的血小板,说明川芎嗪衍生物改性丝素材料的体外抗凝血活性有所改善.更多还原

【Abstract】 In order to improve the antithrombogenicity of silk fibroin(SF),chuanxiongqin(tetramethyl pyrazine,TMPZ),which is an active ingredient of Chinese herbal medical-Ligusticum chuanxiong Hort,was used to modify SF in this paper. C=C was introduced into the side group of TMP,and then they were grafted onto SF surface without initiators.The resulting products were characterized by the attenuated total reflectance Fourier transform infrared spectroscopy(ATR-FTIR), Fourier transform infrared spectroscopy(FTIR),and scanning electron microscopy(SEM).Their antithrombogenicity in vitro was evaluated by the activated partial thromboplastin time(APTT) and platelet adhesion assay.It shows that grafted SF has the longer APTT than SF.Furthermore,blood contacting surface of modified SF does not display any platelet attachment after being incubated in PRP for 30 min.On the contrary,SF surface exhibites a large number of adhered platelets.So it concludes that TMP-modified-SF materials show better antithrombogenicity than SF.The study provides a good example for improving SF with Chinese herb to prepare antithrombotic materials.更多还原