【摘要】 以ω-溴苯乙酮作为脂肪酸衍生剂,利用超高效液相色谱(UPLC)法测定双低菜籽油和结构脂质中脂肪酸组成与位置分布,探讨了流动相、柱温对脂肪酸衍生物分离检测效果的影响。经优化确定以(甲醇-乙腈)(90∶10)-水为流动相梯度洗脱,在检测波长246 nm,柱温35℃,流速0.35mL/min的色谱条件下,18 min内能完成对13种脂肪酸衍生物的分离检测。UPLC法具有操作简单,分析检测时间短,溶剂消耗少,分离度好的优点。更多还原

【Abstract】 A method was developed for the determination of fatty acids positional distribution in triacylglycerols of double-low rapeseed oil and structured lipid using ω-bromoacetophenone as derivative reagent by ultra-high performance liquid chromatography(UPLC).Different chromatographic parameters including elution profiles,column temperature were investigated in order to optimize separation conditions of different fatty acid phenacyl ester derivatives.A gradient elution using methanol/acetonitrile(90∶10,V/V)-water as mobile phase at 0.35 mL/min(flow velocity),detection wavelength at 246 nm,and column temperature of 35 ℃ were chosen.Under these conditions,13 derivatives of fatty acids were separated in 18 min.This method was simple,fast,little solvent-consuming and good separation degree.更多还原