【摘要】 目的改进大鼠血管平滑肌细胞(VSMC)的培养方法。方法采用机械刮除、差异贴壁等手段进行组织贴块法原代培养,胰蛋白酶消化传代,并应用相差显微镜和即用型SABC免疫组化染色试剂盒对细胞进行形态学和免疫组化鉴定。结果镜下培养细胞呈典型的"谷-峰状"生长,免疫组化染色显示胞浆内α-actin阳性表达。结论本法可获纯度高、结构和功能良好的VSMCs。更多还原

【Abstract】 Objective To improve the culture method of vascular smooth muscle cells(VSMCs)in rats.Methods The primary culture and subculture were done by modified tissue-piece inoculation and trypsin digestion respectively.The cells were purified by the mechanical treatment and differential attachment.The cultured cells were identified by morphology and immunohistochemistry with phase contrast microscope and SABC kit.Results The cultured cells possessed typically "peak and valley"characteristics for VSMCs under microscope and expressed the smooth muscle specific differentiation marker smooth muscle-α-actin(SM-α-actin)by immunohistochemical staining.Conclusion The method presented here could obtain the highly purified VSMCs with good structure and function.更多还原