【摘要】 目的建立小儿银牛颗粒中绿原酸的HPLC含量测定方法。方法采用HPLC法,色谱柱为Phenomenex ODSC18色谱柱(4.6mm×250mm,5μm),柱温20℃,流动相为甲醇-0.4%磷酸水溶液(20:80),流速1mL·min-1,检测波长为327nm。结果绿原酸在0.284～1.704μg范围内呈良好的线性关系(r=0.9996),平均回收率和RSD分别为96.19%和1.16%(n=5)。结论该方法操作简便、准确、重现性好,绿原酸得到较好分离,可用于小儿银牛颗粒的质量控制。更多还原

【Abstract】 Objective To develop an HPLC method for the determination of chlorogenic acid in Xiao’er Yinniu Granula.Methods A Phenomenex ODS C18 column (4.6 mm×250 mm,5 μm) was used,column temperature was at 20 ℃,methanol-0.4 %phosphoric acid water solution(20:80)was used as the mobile phase,the flow rate was 1 mL·min-1,and the detection wavelength was 327 nm.Results The calibration curve of chlorogenic acid was linear between 0.284～1.704 μg (r=0.999 6),the average recovery and the relative standard deviation were 96.19 %and 1.16 %(n=5),respectively.Conclusion The method is simple,accurate and reproducible,by whihc chlorogenic acid can be better separated from Xiao’er Yinniu Granula.This method can be used for quality control of Xiao’er Yinniu Granula.更多还原