【摘要】 目的建立胡椒根药材中胡椒碱的含量测定方法。方法采用反相高效液相色谱法,色谱柱:Luna C18柱(250mm×4.60mm,5μm);流动相:甲醇-水(77∶23);流速:1.0mL·min-1;检测波长为343nm;柱温:35℃。结果胡椒碱在0.164μg～0.984μg范围内,具有良好的线性关系,r=0.9996;平均回收率为98.09%,RSD=2.67%(n=9)。三批胡椒根药材胡椒碱含量为6.67～6.77mg·g-1。结论胡椒根含有胡椒碱,该含量测定法可用于控制胡椒根药材的质量。更多还原

【Abstract】 Objective To develop a RP-HPLC method for the determination of piperine in the root of Piper nigrum L.Methods RP-HPLC was carried out on Luna C18 column(250 mm× 4.60 mm,5 μ m) with the column temperature of 35 ℃.The mobile phase consisted of a mixture of methanol-water(77 :23) at a flow rate of 1.0 mL· min-1.The determination wavelength was at 343 nm.Results The calibration curve was linear within the concentration range of 0.164 μ g～ 0.984 μ g,r=0.9996,and the average recovery was 98.09 %,RSD=2.67 %(n=9).The average content of piperine in three batches of pepper roots was in the range of 6.67～6.77mg·g-1.Conclusion Pepper root contains piperine,and this method is suitable for the quality control of the root of Piper nigrum L.更多还原