【摘要】 目的探讨超小型超顺磁性氧化铁颗粒(USPIO)小鼠胰腺增强磁共振成像方法。方法BALb/c小鼠23只,分A、B、C、D四组,每组5只,另外1只小鼠用来研究小鼠胰腺的解剖位置;2只用来注射USPIO前后自身对比。A、B两组确定麻醉剂量后,C、D两组分别为注射USPIO和正常对照组,均采用GESignaHorizonLX1·5T双梯度磁共振仪行双回波序列扫描并建立图像定量分析方法,并使用USPIO增强MRI扫描,计算T2值并观察注射USPIO前后胰腺区域影像学改变。结果使用1·5T磁共振仪及小鼠实验专用线圈在T2序列获得小鼠横断面、矢状位、冠状位图像,图像清晰,能分辨各组织器官。增强磁共振可确定小鼠胰腺所在横断面图像中的层面及具体位置。注射USPIO后和未注射USPIO的胰腺的区域,T2值比较差异有统计学意义(P<0·05)。结论超小型USPIO小鼠胰腺增强MR扫描为检测小鼠胰腺胰岛炎奠定方法学基础。更多还原

【Abstract】 Objective To study imaging of pancreas in mice with ultrasmall superparamagnetic iron oxide(USPIO) -enhanced MRI. Methods Twenty mice were randomly divided into four groups(A,B,C,D) ,besides,1 mouse was used to study the anatomical location of the pancreas,while pancreases of the other two were compared before and after injection of USPIO. After the dose of narcotic determined in group A and B,mice in group C and D were injected USPIO and control group. All mice underwent paired echo sequence using USPIO-enchanced MRI/enchanced MRI with 1.5T MR scanner. The T2 value changes of mice pancreas were evaluated with paired echo sequence with USPIO-enchanced MRI. Results The images of transverse,sagital and coronal of abdomen in mice were achieved clearly with spin sequence,and most of celiac organs of mice could be indentified. The pancreas in mice was accurately located in transverse image. Statistical difference of T2 was found before and after injecting USPIO(P<0.05) . Conclusion USPIO-enhanced MR technique for imaging is the basis of studying pancreatic islet inflammation of mice.更多还原