【摘要】 目的:建立HPLC法同时测定地氯滴耳液中主要成分氯霉素和地塞米松磷酸钠含量的方法。方法:采用依利特C₁₈（250mm×4.6mm,5μm）为色谱柱,流动相为甲醇-乙腈-水（60:10:30）,流速0.5 ml·min^-1,检测波长240nm。结果:氯霉素检测浓度在25.03～250.33μg·ml^-1范围内线性关系良好（r=0.999 9）,平均回收率为99.92%（RSD=0.21%）;地塞米松磷酸钠在5.01～50.13μg·ml^-1范围内线性关系良好（r=0.999 8）,平均回收率为99.48%（RSD=0.61%）。结论:本法快速、准确,可作为该制剂含量测定方法。更多还原

【Abstract】 Objective:To establish a method for the simultaneous determination of chloramphenicol and dexamethasone sodium phosphate in Dilv Auristilla by HPLC.Method:The colum was YiLiTe C₁₈（250mm×4.6mm,5μm）.The mobile phase was methanol-actetitrile -water（60:10:30）,The flow rate was 0.5 ml·min^-1and detection wave length was 240nm.Result:The linear range of chloramphenicol was 25.03-250.33μg·ml ^-1（r=0.999 9）and that of dexamethasone sodium phosphate was 5.01-50.13μg·ml^-1 （r=0.999 7）.The sample’s recoveries were 99.92%,RSD=0.21%and 99.48%,RSD=0.61%respectively（n=3）.Conclusion: This method is rapid,simple,and accurate,which is suitable for the content of this preparation.更多还原