【摘要】 目的:探讨柿叶水提物对H₂₂荷瘤小鼠和S₁₈₀荷瘤小鼠的抗肿瘤作用。方法:建立H₂₂荷瘤小鼠和S₁₈₀荷瘤小鼠移植瘤模型,观察分析柿叶水提物各组对荷瘤小鼠的生命延长率、抑瘤率、胸腺指数、脾脏指数、肿瘤坏死因子（TNF-α）、超氧化物歧化酶（SOD）、丙二醛（MDA）、一氧化氮（NO）的影响。结果:柿叶水提物高、中、低剂量组及环磷酰胺组对H₂₂荷瘤小鼠的生命延长率分别为34.05%,21.08%,5.95%,51.89%,对S₁₈₀荷瘤小鼠的生命延长率分别为30.11%,17.74%,4.30%,45.16%。柿叶水提物高、中、低剂量组及环磷酰胺组的抑瘤率分别为42.08%,25.04%,23.95%,72.77%。柿叶水提物高剂量组的胸腺指数为（3.9±1.0）mg·g^-1,较氯化钠溶液组（3.0±1.1）mg·g^-1提高（P<0.05）,柿叶水提物高剂量组的脾脏指数为（11.8±1.9）mg·g^-1,较氯化钠溶液组（9.2±1.6）mg·g^-1高（P<0.05）。柿叶水提物高、中、低剂量组TNF-α数值分别为（1.94±0.20）,（1.89±0.54）, （1.32±0.18）ng·ml^-1,较氯化钠溶液组（1.18±2.0）ng·ml^-1高（P<0.05）。柿叶水提物高、中、低剂量组SOD数值分别为（312.4±15.06）,（304.1±16.1）,（301.1±19.22）U·ml^-1,较氯化钠溶液组（278.1±11.02）U·ml^-1高（P<0.01或P<0.05）。柿叶水提物高、中、低剂量组MDA数值分别为（5.85±1.12）,（6.31±1.17）,（6.62±1.03）nmol·ml^-1,较氯化钠溶液组（7.26±0.62）nmol·ml^-1下降（P<0.01或P<0.05）。柿叶水提物高、中、低剂量组NO数值分别为（47.0±7.46）,（51.2±9.07）,（56.1±9.98）μmol·L^-1,较氯化钠溶液组（62.2±1.22）μmol·L^-1下降（P<0.01或P<0.05）。结论:柿叶水提物有一定的抗肿瘤作用,其作用机制可能与调节细胞因子TNF-α的分泌、增强免疫功能和抗氧化有关。更多还原

【Abstract】 Objective:To study the antitumor effects of water extract of persimmon leaves in H₂₂-bearing mice and S₁₈₀-bearing mice.Method:H₂₂-bearing mice and S₁₈₀-bearing mice were established and divided into five groups:normal saline（NS） group,cyclophosphamide （CTX） group,high,middle and low dose of water extract of persimmon leaves groups.The life prolongation rate, tumor inhibition rates,values of thymus index,spleen index,Tumor necrosis factor-α（TNF-α）,superoxide dismutase（SOD）,maleic dialdehyde（MDA） and nitrogen monoxidum（NO） were recorded,observed and analyzed.Result:The life prolongation rate of H₂₂-bearing mice in high,middle,low dose groups and CTX group was 34.05%,21.08%,5.95%and 51.89%,respectively,and,the life prolongation rate of S₁₈₀-bearing mice in high,middle,low dose groups and CTX group was 30.11%,17.74%,4.30%and 45.16%respectively.The tumor inhibition rates in high,middle,low dose groups and CTX group were,42.08%,25.04%, 23.95%and 72.77%,respectively.The thymus index was significantly higher in high dose groups than that of in NS group[（3.9±1. 0）mg·g^-1 vs.（3.0±1.1）mg·g^-1,P<0.05],corresponsively,the spleen index of high dose group was higher than that of NS group [（11.8±1.9）mg·g^-1 vs.（9.2±1.6）mg·g^-1,P<0.05].TNF-αof high,middle and low dose groups[（1.94±0.20）ng·ml^-1, （1.89±0.54） ng·ml^-1 and（1.32±0.18）ng·ml^-1]were higher than that of NS group[（1.18±2.0） ng·ml^-1]（P<0.05）.SOD of high,middle and low dose groups[（312.4±15.06）U·ml^-1,（304.1±16.1） U·ml^-1,（301.1±19.22） U·ml^-1]were higher than that of NS group[（278.1±11.02） U?ml^-1]（P<0.01 or P<0.05）.Corresponsively,MDA was lower in high,middle and low dose groups[（5.85±1.12）nmol·ml^-1,（6.31±1.17） nmol·ml^-1 and（6.62±1.03） nmol·ml^-1,respectively]than that in NS group[（7.26±0.62） nmol·ml^-1]（P<0.01 or P<0.05）.NO was lower in high,middle and low dose groups[（47.0±7.46）μmol ?L^-1,（51.2±9.07）μmol·L^-1,（56.1±9.98）μmol·L^-1,respectively]than that in NS group[（62.2±1.22）μmol·L^-1]（P<0.01 or P<0.05）.Conclusion:Water extract of persimmon leaves possess certain antitumor effects,and its mechanism may be related to the effects of adjusting secretion of TNF-α,promoting immune responses and increasing antioxidation.更多还原