【摘要】 目的:探讨清纤方抗肝纤维化的治疗机制。方法:利用CCl4制备肝纤维化大鼠模型,分为正常组、模型组、清纤方组、秋水仙碱组。治疗8周后,用HE和Masson染色观察肝纤维化的形成,免疫组化和RT-PCR检测肝组织中TGF-1βmRNA和蛋白的表达。结果:TGF-β1蛋白和mRNA的表达在治疗组均明显减少。结论:清纤方能有效减轻肝纤维化大鼠的肝脏损伤及纤维化程度,其机制可能与抑制肝内TGF-1β的表达有关。更多还原

【Abstract】 Purpose: To explore the therapeutic mechanism of Qingxianfang for anti-hepatic fibrosis.Method: Prepare hepatic fibrosis models of bandicoots and divide them into groups marked "normal","control","Qingxianfang" and "colchicines" by making use of CCl₄.After eight weeks of medical treatment,an observation of hepatic fibrosis is carried on by using HE and Masson pigmentation,immunohistochemistry and RT-PCR checking of TGF-β₁,mRNA and protein expression in hepatic tissue.Result: The expression of TGF-β₁ protein and mRNA reduced clearly in treatment group.Conclusion: Qingxianfang is effective for easing the liver damage and hepatic fibrosis condition of bandicoots.The mechanism may be relative to the restraining of TGF-β₁ expression inside the liver.更多还原