【摘要】 目的:研究扶正解毒汤(FJD)对硫酸镍(NiSO4)诱导大鼠肾细胞系(NRK)微核形成的抑制作用。方法:以NiSO4、FJD含药血清处理NRK细胞,检测微核率的变化及细胞总的清除自由基抗氧化力(TRAC)。结果:NiSO4(400μmol/L)暴露组细胞微核率、TRAC明显下降,低、中、高剂量FJD血清与NiSO4共处理组细胞微核率显著低于NiSO4暴露组(P<0.05,P<0.01),细胞TRAC则显著提升(P<0.05,P<0.01)。结论:NiSO4可诱发细胞微核形成,FJD对镍诱导NRK细胞微核形成有明显抑制作用,其机制可能与抗氧化应激有关。更多还原

【Abstract】 Objectives: To study the inhibitory effect of Fuzheng Jiedu Decoction（FJD） on the micronuclei formation induced by nickel sulfate（NiSO₄） in rat renal cell line（NRK）.Methods:Cultured NRK cells were exposed to NiSO₄（400μmol/L） and FJD drug serum.Micronuclei（MN） were scored in 1000 NRK cells and cellar total radical-trapping antioxidative capacity（TRAC）was measured.Results: Compared with NiSO₄ group,the MN frequencies decreased and TRAC increased in groups treated with both FJD and NiSO₄ significantly（P<0.05,P<0.01）.Conclusion: FJD could suppress nickel-induced micronuclei formation in NRK cells which was probably related to its antioxidant property.更多还原