【摘要】 目的:采用4种信号转导阻断剂研究5种天然药物姜黄素(Curcumin,Cur)、榄香烯(elemene,Ele)、三氧化二砷(arsenite trioxide,As2O3)、雷公藤内酯醇(triptolide,Tri)、莪术(rhizoma curcumae,Rc)抑制重组人碱性成纤维细胞生长因子(recombinant human basic fibroblast growth factor,rhbFGF)所诱导的牛晶状体上皮细胞(human lens epithelial cell,LEC)增殖的细胞信号转导机制。方法:将rhbFGF诱导体外培养的LEC发生增殖,用Cur、Ele、As2O3、Tri、Rc分别与其共同孵育,并用4种细胞信号转导阻断剂H7、PD98059、W7、尼卡地平(Nicardip-ine)分别作用于上述LEC。用流式细胞术(flow cytometer,FCM)检测LEC增殖细胞核抗原(proliferating cell nu-clear antigen,PCNA)的蛋白表达。结果:增殖组LEC的PCNA蛋白表达显著高于对照组(P<0.01);Cur组、Ele组、As2O3组、Tri组、Rc组的PCNA均比增殖组显著降低(P<0.01);Cur抑制LEC增殖的信号转导通路可被H7、PD98059和nicardipine阻断;Ele和As2O3均可被H7和W7阻断;Tri可被W7、nicardipine阻断;Rc可被H7、W7、PD98059和nicardipine阻断。以上均有显著性差异(P<0.01)。结论:5种天然药物Cur、Ele、As2O3、Tri、Rc抑制LEC增殖的作用是通过蛋白激酶C、丝裂原活化蛋白激酶、Ca2+-钙调蛋白、钙通道途径等信号转导机制,为寻求防治后发性白内障的有效药物提供了科学依据。更多还原

【Abstract】 Objective: To study the mechanism of proliferation inhibited by five natural medicines in lens epithelial cell via blockers of signal transduction.Methods: The proliferations of cultured LEC were induced by rhbFGF,which were incubated with five natural medicines,Curcumin(Cur),elemene(Ele),arsenite trioxide(As2O3),triptolide(Tri) and rhizoma curcumae(Rc) respectively.Meanwhile the LEC were incubated with four blockers of signal transduction,H7、W7、PD98059和nicardipine respectively.The proliferating cell nuclear antigen(PCNA)of LEC were detected via flow cytometer(FCM).Results:The PCNA expression of LEC in proliferation group were higher than that in control group(P<0.01),there were decreased in groups Cur,Ele,As2O3,Tri and Rc significantly(P<0.01).The signal pathways of proliferation inhibited by Cur can be blocked by H7,PD98059和nicardipine.It can be blocked by H7and W7in group Ele and groups As2O3,can be blocked by W7and nicardipine in group Tri,can be blocked by H7,W7,PD98059 and nicardipine in group Rc.Conclusion: The mechanism of LEC proliferation inhibited by five natural medicines,Cur,Ele,As2O3,Tri and Rc,may be the signal pathways of protein kinase C(PKC),mitogen activated protein kinase(MAPK),Ca2+-calmodulin(CaM) and calcium channel,which provided the scientific basis for pursuit of effective medicine to prevent and treat after cataract.更多还原