【摘要】 目的:研究三七总皂苷(PNS)对大鼠肾小球系膜细胞(MCs)/CTGF表达及细胞外基质(ECM)产生的影响。方法:使用高、中、低浓度(400、200、100μg/mL)的PNS预处理大鼠MCs30min,再加入终浓度为10-6mol/L血管紧张素Ⅱ(AngⅡ)共孵育48h。实验终点采用RT-PCR法测定细胞内(CTGF mRNA的表达;采用放免法测定培养上清液中Ⅳ型胶原(ColⅣ)、纤维连接蛋白(FN)的含量;然采用ELISA法测定培养上清液中层黏蛋白(LN)的含量。结果:各浓度的PNS均能显著抑制AngⅡ诱导的大鼠MCsCTGF mRNA表达(P<0.05),并能抑制AmgⅡ诱导的大鼠MCsⅣ型胶原、FN以及LN的分泌(P<0.05或P<0.01),且以400μg/m LPNS的作用最强。结论:PNS能通过抑制MCs CTGF的表达以及分泌细胞外基质,从而抑制一系列致肾小球硬化作用,在慢性肾脏疾病的治疗中具有一定的应用价值。更多还原

【Abstract】 Objective:To investigate Effect of PNS on the expression of CTGF of rat MCs and secretion of ECM. Methods:Rat MCs are forcecultured with 400μg/mL,200μg/mL,100μg/mL PNS and just RPMI-1640 for 30 minutes,then cultured for 48 hours after adding 10~6mol/L AngⅡ.On the end point,laminin (LN) are detected by ELISA;CTGF mRNA is deected by RT-PCR assay;type Ⅳ collagen (Col Ⅳ) and fibronectin (FN) are detected by radioimmunity(RI).Results:All PNS groups can significantly inhibit expressioin of CTGF mRNA in CMs induced by AngⅡ(P<0.05),and All PNS groups can significantly inhibit MCs expressing CTGF mRNA and secretting ColⅣ,LN and FN.Accordingly,PNS may can delay or prevent glomerulosclerosis by these effects.It is possible to provid a effective drug for treatment of chronic kidney diseases.更多还原