【摘要】 目的探讨人参瓜蒌莪术汤对lewis肺癌模型小鼠的治疗作用及机制。方法建立小鼠Lewis肺癌移植瘤模型,分为模型对照组(NS组),环磷酰胺对照组(CP组),人参瓜蒌莪术汤高剂量组(RGEH组)、中剂量组(RGE组)、低剂量组(RGEL组)。在计算抑瘤率的基础上,运用TUNEL法原位检测瘤组织细胞凋亡。结果人参瓜蒌莪术汤对小鼠Lewis肺癌表现出明显的抑制作用,中剂量组效果最为明显,抑瘤率可达47.88%;人参瓜蒌莪术汤低、中、高剂量组均能明显诱导瘤组织细胞凋亡(P<0.01)。中、高剂量组诱导细胞凋亡作用强于环磷酰胺阳性对照组(P<0.05)。结论人参瓜蒌莪术汤具有抑制小鼠Lewis肺癌生长的作用,其作用机制可能与诱导肿瘤组织细胞调亡有关。更多还原

【Abstract】 Objective To explore the influence of treatment and mechanism by Renshengualouezhu Decoction (RGE) to Lewis lung cancer in mice.Methods With international standard model for tumor study - Lewis lung cancer in C57BL/6 Mice model,they calculated the inhibition rate and used TUNEL to detect the Apoptosis of tumor cells.Fifty C57BL/6 Mice were divided into 5 groups;control group,low - dose RGE group,middle—dose RGE group,high - dose RGE group and Cyclophosphamide(CP) group.Results Low,middle and high dose RGE group and CP group tumor weight compared with the control group were significantly lower(P < 0.01 ).Inhibition rate in the middle - dose RGE group reached 47.88%.RGE can deduce the Apoptosis of tumor cells on all dose levels - Low,Medium or High(P < 0.01).Medium and High groups showed better effect the Cyclophosphamide comparison group(P < 0.05).Conclusion RGE can significantly restrict Mice Lewis lung cancer.Its bio - molecular mechanism might be related to its ability to induced tumor cell apoptosis.更多还原