Effect of Quyu Jiedu Granule(祛瘀解毒颗粒) on Micro-environment of Ova in Patients with Endometriosis

【摘要】 <正>Objective:To observe the effect of Quyu Jiedu Granules(祛瘀解毒颗料,QJG) on the micro-environment of ova in patients with endometriosis(EM).Methods:Twenty EM patients who received in vitro fertilization and embryo transfer(IVF-ET) were randomized equally into a treated group and a control group. Further,20 patients who received IVF-ET due to oviduct factors were enrolled into a non-endometriosis group. The dosage of gonadotrophic hormone used,the number of ova attained,fertilization rate and clinical pregnancy rate were all observed,and the levels of tumor necrosis factorα(TNF-α) and interleukin 6(IL-6) in follicular fluid as well as their mRNA expressions in ovarian granular cells were detected by RT-PCR on the very day of ovum attainment.Results:The ova attainment(13.80±6.87) and fertilization rate(0.69±0.31) in the treated group were all higher than the corresponding values in the control group(9.80±5.32 and 0.47±0.22);the follicular fluid contents of TNF-αand IL-6 in the treated group were 1.38±0.21 ng/mL and 130.56±12.81 pg/mL,respectively,which were lower than those in the control group(1.98±0.34 ng/mL and 146.83±17.65 pg/mL,respectively).Further,the treated group showed much lower mRNA expressions of TNF-αand IL-6 in ovarian granular cells.Conclusions:The elevation of TNF-αand IL-6 contents in follicular fluid and their mRNA expressions in ovarian granular cells are possibly related to the low quality of ova in EM;QJG might raise the ova quality by reducing TNF-αand IL-6 levels to improve the living micro-environment for the ova.更多还原

【Abstract】 Objective:To observe the effect of Quyu Jiedu Granules(祛瘀解毒颗料,QJG) on the micro-environment of ova in patients with endometriosis(EM).Methods:Twenty EM patients who received in vitro fertilization and embryo transfer(IVF-ET) were randomized equally into a treated group and a control group. Further,20 patients who received IVF-ET due to oviduct factors were enrolled into a non-endometriosis group. The dosage of gonadotrophic hormone used,the number of ova attained,fertilization rate and clinical pregnancy rate were all observed,and the levels of tumor necrosis factorα(TNF-α) and interleukin 6(IL-6) in follicular fluid as well as their mRNA expressions in ovarian granular cells were detected by RT-PCR on the very day of ovum attainment.Results:The ova attainment(13.80±6.87) and fertilization rate(0.69±0.31) in the treated group were all higher than the corresponding values in the control group(9.80±5.32 and 0.47±0.22);the follicular fluid contents of TNF-αand IL-6 in the treated group were 1.38±0.21 ng/mL and 130.56±12.81 pg/mL,respectively,which were lower than those in the control group(1.98±0.34 ng/mL and 146.83±17.65 pg/mL,respectively).Further,the treated group showed much lower mRNA expressions of TNF-αand IL-6 in ovarian granular cells.Conclusions:The elevation of TNF-αand IL-6 contents in follicular fluid and their mRNA expressions in ovarian granular cells are possibly related to the low quality of ova in EM;QJG might raise the ova quality by reducing TNF-αand IL-6 levels to improve the living micro-environment for the ova.更多还原