【摘要】 [目的]观察中性粒细胞趋化因子(CINC)在重症急性胰腺炎(SAP)早期肺组织中表达的动态变化,探讨清胰汤(QYT)对SAP肺损伤(ALI)的保护作用及其机制。[方法]采用大鼠胰腺被膜下均匀注射5%牛磺胆酸钠0.4ml/100g制作SAP模型,随机分为假手术(SO)组,SAP组和QYT组。SAP模型制作后立即给予QYT1ml/kg灌胃,每8h重复1次。后2组又各分为6、12、24h3小组,总计7组,每组8只。观察肺组织病理学改变,肺湿重(W)/干重(D)比率,髓过氧化酶(MPO)活性,肿瘤坏死因子α(TNF-α)浓度和CINC mRNA的表达。[结果]SO组肺组织CINC mRNA有轻度表达;SAP6、12、24h各组CINC mRNA表达明显高于SO组,24h组达高峰(P<0.01),与肺W/D比率、MPO活性、TNF-α浓度及肺组织病理变化相关(P<0.05)。与SAP组比较,QYT组肺W/D比率、MPO活性和TNF-α浓度明显下降,CINC mRNA表达降低(P<0.05),肺组织病理学损害减轻,QYT24h组肺组织病理学损害减轻显著(P<0.05)。[结论]CINC是大鼠SAP早期ALI重要的炎症递质,QYT能抑制大鼠SAP肺组织中CINC的表达,从而减轻大鼠SAPALI。更多还原

【Abstract】 [Objective]To investigate the expression of the CXC chemokine cytokine-induced neutrophil chemoattractant (CINC)in severe acute pancreatitis (SAP)and to examine the effects of QingYiTang(QYT)on the course of pancreatitis-associated acute lung injury (ALI).[Methods]SD rats induced by homogeneous injection of 0.4ml/㎏ of 5 percent sodium taurocholate beneath the pancreatic capsule were treated with QYT.After 6,12 and 24 hours,tissue edema was deter-mined by wet/dry weight ratios and myeloperoxidase(MPO)activity of lungs were measured;pathological changes in the pancreas and lungs were observed;expression of CINCmRNA and TNF-αconstration in lungs were determined by reverse transcriptase polymerase chain reaction (RT-PCR)or enzyme-linked immunosorbent assay (ELISA).[Results]Compared with that in shams,wet/dry weight ratios,pulmonary expression of CINC gene,MPO activity,TNF-αconcentrations in the lungs and the serum amylase were increased greatly in 24 hours(P<0.05).The CINCmRNA expression in lungs was significantly correlated with the severity of lung injury,MPO activity and TNF-αconcentrations in the lungs(P<0.05).administration of QYT significantly decreased the elevation of MPO activity,wet/dry weight ratios ,TNF-αconcentrations and CINCmRNA expression in the lung(P<0.05),and improved the histological findings(P<0.05).[Conclusion]Overexpression of CINC mRNA in the lungs might play an important role in the pathogenesis of ALI in SAP.QYT reduced the severity of ALI,suggesting a possible application of this compound in the treatment of acute pancreatitis.更多还原