【摘要】 目的:探讨铁超载和脂肪堆积在二甲基亚硝胺(Dimethylnitrosamine,DMN)诱导的肝纤维化大鼠组织病理学变化中的作用。方法:模型组大鼠(n=30)腹腔注射DMN,10μl.kg-1.d-1,每周连续3日,共4周。模型A、B组大鼠分别于注射DMN完毕后的第1日、第21日处死。对照组大鼠(n=10)腹腔注射等体积生理盐水。各组大鼠肝组织分别予HE染色、Masson染色及普鲁士蓝染色,电镜下观察。结果:伴随着胶原生成的铁沉积于肝小叶是腹腔注射DMN完毕后的第1日、第21日肝组织的显著特点。但肝细胞脂肪堆积仅仅发生在腹腔注射DMN完毕后的第21日。结论:铁超载和脂肪堆积可能在DMN诱导的大鼠肝纤维化病理变化中发挥重要作用,其具体机制有待进一步研究。更多还原

【Abstract】 Objective:To investigate the histopathology of iron overloading and fat accumulation in dimethylnitrosamine(DMN)-induced liver fibrosis in rats.Methods:Thirty rats were treated with DMN at does consecutive days of 10μl·kg-1·d-1,i.p.for 3 consecutive day each week for 4 wk.Rats(n=30)were sacrificed on the first day(model group A)and 21st d(model group B)after cessation of DMN injection.The control group(n=10)received an equivalent amount of normal saline.Liver tissues were stained with hematoxylin & eosin(HE)and Masson and Prussian blue staining and oberserved under electron microscopy.Results:The liver fibrosis did not automatically reverse,which was similar to previous reports,the perilobular deposition of iron accompanied with collagen showed marked characteristics at both the first and 21st d after cessation of DMN injection.However,fat accumulation in hepatocytes occurred only at the 21st d after cessation of DMN injection.Conclusion:Iron overloading and fat accumulation may play important roles in pathological changes in DMN-induced rats liver fibrosis.The detailed mechanisms of these characteristics need further research.更多还原