【摘要】 目的研究Bcl-2基因家族在紫杉醇诱导HL-60细胞凋亡中的表达水平变化。方法DNA凝胶电泳法检测细胞凋亡;RT-PCR法检测在紫杉醇诱导前后Bcl-2基因家族的表达水平。结果紫杉醇诱导HL-60细胞凋亡具有剂量依赖性,结果支持紫杉醇诱导HL-60细胞凋亡有剂量依赖性的观点,同时也观察到了低剂量紫杉醇(10-8)即可诱导Bad基因表达明显增加,高剂量紫杉醇(10-6)才可诱导Bcl-2基因表达明显减弱,但未见Bax基因和Bcl-xl基因表达的明显变化。结论紫杉醇能通过诱导细胞凋亡杀伤HL-60细胞,而此凋亡可能与Bad的表达上调和Bcl-2基因表达下调有关,而与Bax和Bcl-xl的表达无关。更多还原

【Abstract】 【Objective】To study the changes of Bcl-2 gene family expression levels in the process of paclitaxel-induced apoptosis on HL-60 cells.【Methods】DNA gel electrophoresis was used to demonstrate DNA fragmentations.Reverse transcriptase polymerase chain reaction(RT-PCR) was used to detect Bcl-2 expression levels.【Results】Paclitaxel-induced apoptosis on HL-60 cells has a dose-dependent quality.Meanwhile,according to the RTPCR results,we have observed that the low dose of paclitaxel(10-8) could induce the increasing of the Bad gene expressions obviously.While,the high dose of paclitaxel(10-6) could obviously induce the decreasing of the Bcl-2 gene expressions.But we haven’t observed the obvious changes of expressions of Bax gene and Bcl-xl gene.【Conclusions】Paclitaxel could kill the HL-60 cells by inducing apoptosis.And it were possibly related with Bad upregulation expression and Bcl-2 downregulate expression,and irrelevant with Bax expression level and Bcl-xl expression level.更多还原