【摘要】 目的探讨内皮素-1(Endothelin 1,ET-1)对食管鳞状上皮细胞癌细胞侵袭作用的影响。方法用Western blotting法检测75例食管鳞癌组织及配对正常食管粘膜上ET-1的蛋白表达,并用双重ET受体抑制剂Bosentan进行干预,用Matrigel体外侵袭试验显示食管癌细胞侵袭能力。结果Western blotting法显示74.7%(56/75)的食管癌组织中有ET-1蛋白高表达;Bosentan抑制ET-1与其受体结合并降低其生物作用,显著抑制食管癌细胞的体外侵袭。结论ET-1可增强人类食管鳞癌细胞的侵袭力。更多还原

【Abstract】 Objective To explore the effects of endothelin-1 on invasion of squamous cell carcinoma of esophagus.Methods Western Blotting were employed to detect the expression of ET-1 protein in 75 samples with squamous cell carcinoma of esophagus and matched normal esophageal mucous membrane, and intervention with bosentan, a dual ET(A/B)-receptor antagonist.Matrigel invasion assays in vitro were used to show the invasive ability of esophageal cancer cells.Results 74.7%(56/75) tumors had an overexpression of ET-1 protein by Western Blotting.Bosentan inhibited the combination between ET-1 and it′s receptor, and degrade ET-1 activity, significantly inhibited Matrigel invasion of cancer cells in vitro.Conclusion Endothelin-1 can increase the invasive ability of human esophageal cancer cells.更多还原