【摘要】 目的探讨格列卫(Gleevec or Glivec,代号STI571)在体外对人胃肠道间质瘤-T1细胞(GIST-T1)血管内皮生长因子(VEGF)表达的影响。方法体外培养人胃肠道间质瘤-T1细胞,MTT法检测格列卫对人胃肠道间质瘤-T1细胞的增殖抑制作用,RT—PCR方法检测VEGF mRNA表达,Western blot检测VEGF蛋白表达水平的变化。结果格列卫呈剂量性抑制人胃肠道间质瘤-T1细胞;人胃肠道间质瘤-T1细胞高水平表达VEGF,格列卫能显著降低人胃肠道间质瘤-T1细胞VEGF mRNA和蛋白表达。结论格列卫可以下调人胃肠道间质瘤-T1细胞VEGF的表达,进一步抑制人胃肠道间质瘤-T1细胞的增殖。更多还原

【Abstract】 【Objective】 To investigate whether Gleevec could inhibit the vascular endothelial growth factor(VEGF) expression of human gastrointestinal stromal tumor line GIST-T1.【Methods】Human gastrointestinal stromal tumor cell line GIST-T1 was cultured in vitro,proliferation inhibitory effect was tested by MTT assay;VEGF mRNA was measured quantitatively by RT-PCR method,Expression of VEGF protein in GIST-T1 were measured by West-ern blot.【Results】Gleevec could inhibit the proliferation of GIST-T1 cells,with dose-related effects;VEGF mRNA expressed in high level in GIST-T1 cells,in which,Gleevec significantly decreased the expression of VEGF mRNA and protein.【Conclusion】Gleevec could inhibit proliferation and lower the expression of VEGF in human gastroin-testinal stromal tumor cell line GIST-T1.更多还原