【摘要】 目的研究弓形虫感染昆明种及Babl/c小鼠的病理特征。方法分别以刚地弓形虫速殖子感染昆明种及Babl/c小鼠,观察小鼠感染发病情况,并用病理组织切片,直接染色或免疫组化法检测小鼠体内弓形虫包囊或抗原。正常对照组小鼠未感染弓形虫。结果两种小鼠对刚地弓形虫均易感,昆明种小鼠感染刚地弓形虫后2、3d开始发病,感染后7～10d死亡,死亡率95.00%;Babl/c小鼠于感染后4、5d开始发病,呈一过性急性发作,绝大多数小鼠能度过急性期逐步恢复而长期存活,仅少数小鼠死亡,死亡率为10.00%。昆明种小鼠感染后主要表现为肝细胞变性、坏死、大量炎细胞浸润,小肠黏膜坏死脱落,腹腔内可见大量弓形虫速殖子;Babl/c小鼠在急性感染期表现为肝细胞水变性,腹腔内可见弓形虫速殖子,度过急性期后继续存活的小鼠体内虽未见到大量包囊、但在肝、脑等组织中可检出弓形虫抗原。对照组小鼠在实验期间无任何异常。结论两种小鼠对刚地弓形虫的易感性不同,Babl/c鼠感染弓形虫后经历了急性和慢性感染的转换过程,是研究弓形虫感染与免疫的良好模型。更多还原

【Abstract】 [Objective]To study the pathological changes in Kunming mice and Babl/c mice infected with toxo-plasma gondii. [Methods] Kunming mice and Babl/c mice were infected with toxoplasma tachyzoite by intraperi-toneal injection. Then, the infection rate and pathological histology were observed in infected mice. The control mice only received an injection of same amount of saline. [Results]The two kind of mice were susceptible to toxoplasma gondii. The Kunming mice showed infection at 2nd-3rd day postinoculated, and died at 7th～10th day postinfection. The mortality was 95.00%. The hepatocyte denaturation and necrosis were observed. And a lot of toxoplasma tachy-zoites could be obtained from abdominal cavity. But in Babl/c mice, the onset of the sickness was at 4th ～ 5th day postinfection. Most mice could live through the acute stage of infection. The mortality was only 10.00%. The toxo-plasma antigen could be detected in mouse liver and brain, Although the cyst could not be found. All control mice were normal in the research time. [Conclusion] The susceptibility of toxoplasma infection in these two kinds of mice were different. The Babl/c mouse probably is a good model to research the toxoplasma infection and immunity.更多还原