【摘要】 目的研究基因纳米复合物对肝癌的靶向性治疗作用。方法使用荧光显微镜检测增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)评价转染效率,RT-PCR法检测Survivin mRNA的表达,流式细胞术检测凋亡率和细胞周期分布。结果基因纳米复合物靶向性好,转染效率高,对肝癌有很好的基因治疗效果。结论基因纳米复合物在肝癌的基因治疗方面有很好的发展前景。更多还原

【Abstract】 【Objective】Research of DNA-Nanoparticle(DNA-NP) targeted therapy for liver cancer.【Methods】 Check EGFP protein by fluorescence microscopy,check expression of Survivin mRNA by RT-PCR,Check cell cycle distribution and apoptosis rates by flow cytometry.【Results】DNA-NP show advantages in target and transfection efficiency,DNA-NP have good effect of gene therapy for liver cancer.【Conclusion】DNA-NP have good foreground of gene therapy for liver cancer.更多还原