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HPLC法测定益康胶囊中VitA、VitE的含量

Determination of VitA and VitE in Yikang Capsule by HPLC

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【作者】 张雪周燕双

【Author】 ZHANG Xue,ZHOU Yan-shuang.Guangzhou wanglaoji Pharmaceutical Company limited,Guangzhou 510450,China

【机构】 广州王老吉药业股份有限公司

【摘要】 目的建立益康胶囊中VitaA和VitE含量测定方法。方法采用高效液相色谱法进行分析,流动相为甲醇-水(92:8),检测波长:VitA325nm、VitE285nm。结果峰面积与含量有良好的线性关系(VitA:0.004969mg~0.159mg,VitE:0.06889mg~2.2046mg);高、中、低3种浓度的平均加样回收率为:VitA98.56%、VitE99.04%。结论本法准确、简便、快速为益康胶囊质量控制提供科学依据。

【Abstract】 Objective To establish a HPLC method for determining the contents of VitA and VitE in Yikang Capsule.Methods An HPLC method was developed,mobile phase:methanol-water(92:8),wavelength of VitA at 325 nm and wavelength of VitE at 285 nm.Results Linearity of VitA was found in the range of 0.004969 mg~0.159 mg,the average recovery of VitA was 98.56%;Linearity of VitE was found in the range of 0.06889 mg~2.2046 mg,the average recovery of VitA was 99.04%.Conclusion This result shows that this method is easy to handle,accurate and with good reproducibility,so it can be used for quality control for Yikang Capsule.

【关键词】 益康胶囊VitAVitE高效液相色谱法
【Key words】 Yikang CapsuleVitAVitEHPLC
  • 【文献出处】 中国现代药物应用 ,Chinese Journal of Modern Drug Application , 编辑部邮箱 ,2009年11期
  • 【分类号】R286
  • 【下载频次】33
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