【摘要】 目的探讨小儿急性阑尾炎细菌移位、一氧化氮(NO)、白介素-10(IL-10)的变化及临床意义。方法应用聚合酶链反应(PCR)定性检测阑尾炎组及对照组全血中的细菌基因;应用酶联免疫吸附试验(ELISA)定量检测阑尾炎组术前、术后7d和对照组血清中NO、IL-10的水平。结果急性单纯性阑尾炎组16SrRNA、大肠杆菌(BG)未检出;化脓性阑尾炎组及坏疽性阑尾炎组16SrRNA阳性率分别为70%、80%,BG阳性率分别为60%、70%;阑尾炎术前组NO浓度与术后组差异有统计学意义(P<0.01);阑尾炎术前组NO浓度与对照组间差异有统计学意义(P<0.01);术后组差异无统计学意义(P>0.05);阑尾炎术前组IL-10浓度与术后组间比较差异有统计学意义(P<0.01);阑尾炎术前组IL-10浓度与对照组间比较差异有统计学意义(P<0.01)。结论小儿急性阑尾炎细菌移位的发生,NO、IL-10起着重要作用,检测其变化对细菌移位的发生机制及临床诊治有一定的价值。更多还原

【Abstract】 Objective To evaluate the acute appendicitis in children with bacterial translocation occurred,appendicitis in children pre-operation and after-operation 7 days of serum NO(nitric oxide)and IL-10(interleukin 10);Methods PCR(polymerase chain reaction)testing as appendicitis and control groups of bacteria in whole blood;Application of ELISA(enzyme-linked immunosorbent assay)quantitative detection of appendicitis group pre-operation and after-operation 7 days and the control group serum levels of NO and IL-10;Result The control group and simple acute appendicitis group in whole blood were not detected bacteria;Purulent appendicitis group and gangrenous appendicitis group 16SrRNA positive rates were 70%,80%,BG positive Rates were 60%,70%;Appendicitis pre-operative group,the concentration of NO was different from the after-operative group,statistically significant(P<0.01);Appendicitis pre-operative group,the concentration of NO was different from the control group,statistically significant(P<0.01);After operative group had no difference,not significant(P> 0.05);Appendicitis pre-operative group,the concentration of IL-10 was different from the after-operative group,statistically significant(P<0.01);Appendicitis pre-operative group,the concentration of IL-10 was different from the control group,statistically significant(P<0.01);After operative group had no difference,not significant(P> 0.05);Conclusion Acute appendicitis in children incides bacterial translocation,detection of NO and IL-10 in children with acute appendicitis play an important role in the mechanism of bacterial translocation and a certain clinical value.更多还原