【摘要】 利用田间试验,以转Bt基因棉花Z30、转双价(Bt+CpTI)棉花SGK321及其相应的等价基因Z16、SY321作为供试对象,研究棉花种植后对土壤水解/氧化还原酶类活性的影响。结果表明,转Bt棉花及转双价棉花的种植对各种土壤酶活性的影响不一致,转Bt棉花及转双价棉花种植显著降低了土壤蛋白酶和脱氢酶活性(P<0.05)。转Bt棉花还显著降低了土壤脲酶活性(P<0.05);转双价棉花种植同时显著降低土壤磷酸二酯酶、β-葡萄糖苷酶、过氧化氢酶和硝酸还原酶活性(P<0.05)。棉花品种及转基因行为(转入基因类型)均未给土壤酸性磷酸单酯酶和FDA活性带来影响;土壤芳基硫酸酯酶所受的影响主要来自于棉花品种自身。更多还原

【Abstract】 With"Z16"and"SY321"as near-isogenic counterparts respectively,soil hydrolase and oxidoreductase activities were studied in a field experiment by planting transgenic Bt cotton"Z30"and transgenic Bt+CpTI cotton SGK321.The results show that there are no consistent influences of transgenic Bt cotton and transgenic Bt+CpTI cotton on each soil enzyme activity.The activities of soil protease and dehydrogenase are both significantly reduced in the two transgenic cottons’ fields(P<0.05).Moreover,the activity of soil urease is significantly reduced in the transgenic Bt cotton field as well.The activities of soil phosphodiesterase,β-glucosidase,catalase and nitrate reducatse are significantly reduced in the transgenic Bt+CpTI cotton field,while those in the transgenic Bt cotton field are not obviously changed.Neither cotton cultivars nor transgnic events(difference of modified genes) have effects on soil acid phospho-monesterase and FDA hydrolysis activities;however,the effect on soil arylsulphatase activity is mainly from the difference of cotton cultivars.更多还原